Hotspot

rpl16

LSC · 80439-81789 · 1351 bp

Back to Frankenia

Pi 0.0104

MarkerSeek score 50.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1351
variable_sites: 14
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGATTGCTAGCGCCTATGAT	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2170-2173 bp	1.0	80.3
rpl16_p2	AAAACGCAAAACACCTGTGG	GTCGAGGGAATTGCACGTAT	45.0	50.0	58.0	58.1	2028-2031 bp	1.0	80.3
rpl16_p3	CGATTGCTAGCGCCTATGAT	TGCGCCTATACAGTTCGAAC	50.0	50.0	58.0	58.1	1753-1756 bp	1.0	80.2
rpl16_p4	TGTCCAAAACGCAAAACACC	GTCGAGGGAATTGCACGTAT	45.0	50.0	58.0	58.1	2033 bp	0.5	59.5
rpl16_p5	CCAAAACGCAAAACACCTGT	GTCGAGGGAATTGCACGTAT	45.0	50.0	58.0	58.1	2030 bp	0.5	59.5