Hotspot

trnL-UAG-ccsA

SSC · 114111-114233 · 123 bp

Back to Frankenia

Pi 0.041

MarkerSeek score 69.50

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 123
variable_sites: 5
pi: 0.041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	ACAATCATTGAAAAAGAAATCTGAGT	50.0	26.9	57.9	56.4	239-240 bp	1.0	42.0
trnL-UAG-ccsA_p2	GTAGACACGCTGCTCTTAGG	ACAATCATTGAAAAAGAAATCTGAGT	55.0	26.9	57.8	56.4	240-241 bp	1.0	42.0
trnL-UAG-ccsA_p3	AAATTGGTAGACACGCTGCT	ACAATCATTGAAAAAGAAATCTGAGT	45.0	26.9	57.8	56.4	246-247 bp	1.0	41.9
trnL-UAG-ccsA_p4	AACAAGCCGCTATGGTGAAA	ACAATCATTGAAAAAGAAATCTGAGT	45.0	26.9	58.1	56.4	263-264 bp	1.0	41.8
trnL-UAG-ccsA_p5	ACAAGCCGCTATGGTGAAAT	ACAATCATTGAAAAAGAAATCTGAGT	45.0	26.9	57.9	56.4	262-263 bp	1.0	41.8