Hotspot

rpl32-trnL-UAG

SSC · 112891-114030 · 1140 bp

Back to Frankenia

Pi 0.0246

MarkerSeek score 64.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1140
variable_sites: 28
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCATCTCTTTCGAAAGCGA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.6	57.9	1228-1296 bp	1.0	78.0
rpl32-trnL-UAG_p2	AGCATCTCTTTCGAAAGCGA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.6	57.8	1221-1289 bp	1.0	77.5
rpl32-trnL-UAG_p3	AGCATCTCTTTCGAAAGCGA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.6	57.8	1227-1295 bp	1.0	77.4
rpl32-trnL-UAG_p4	AGCATCTCTTTCGAAAGCGA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.6	57.8	1226-1294 bp	1.0	77.4
rpl32-trnL-UAG_p5	AGCATCTCTTTCGAAAGCGA	GAGCAGCGTGTCTACCAATT	45.0	50.0	57.6	58.3	1222-1290 bp	1.0	76.8