Hotspot

trnN-GUU-ndhF

IRb · 108086-109536 · 1451 bp

Back to Frankenia

Pi 0.0118

MarkerSeek score 51.00

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1451
variable_sites: 17
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	ATCTCCCCAGGTAGGATTCG	TCCGTTCAACCAACAAGGAA	55.0	45.0	58.0	57.6	2063-2121 bp	1.0	78.0
trnN-GUU-ndhF_p2	ATCTCCCCAGGTAGGATTCG	TTCCGTTCAACCAACAAGGA	55.0	45.0	58.0	57.6	2064-2122 bp	1.0	78.0
trnN-GUU-ndhF_p3	ATCTCACTTCACACCTCGGA	TCCGTTCAACCAACAAGGAA	50.0	45.0	58.1	57.6	2737-2846 bp	1.0	77.4
trnN-GUU-ndhF_p4	ATCTCACTTCACACCTCGGA	TTCCGTTCAACCAACAAGGA	50.0	45.0	58.1	57.6	2738-2847 bp	1.0	77.4
trnN-GUU-ndhF_p5	TCTTAACCCGGAAATGGCTG	TCCGTTCAACCAACAAGGAA	50.0	45.0	57.9	57.6	2678-2787 bp	1.0	77.0