Hotspot

trnR-ACG-trnN-GUU

IRb · 107553-108013 · 461 bp

Back to Frankenia

Pi 0.0174

MarkerSeek score 65.80

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 461
variable_sites: 8
pi: 0.0174
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	602-653 bp	1.0	79.3
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	553-604 bp	1.0	78.1
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TTCGAATCCTACCTGGGGAG	55.0	55.0	57.8	58.2	543-594 bp	1.0	77.7
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	CCAATCTCCCGTTGTTCCTC	55.0	55.0	57.8	58.3	610-661 bp	1.0	77.2
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	55.0	50.0	57.8	57.7	557-608 bp	1.0	77.2