Hotspot

trnS-GGA-rps4

LSC · 44481-44873 · 393 bp

Back to Frankenia

Pi 0.0148

MarkerSeek score 65.00

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 393
variable_sites: 5
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	GATAGGAGAGATGGCCGAGT	TTGACTCTTCAGCCATTCCC	55.0	50.0	58.1	57.5	546-601 bp	1.0	80.1
trnS-GGA-rps4_p2	TGATAGGAGAGATGGCCGAG	TTGACTCTTCAGCCATTCCC	55.0	50.0	58.1	57.5	547-602 bp	1.0	80.1
trnS-GGA-rps4_p3	GATAGGAGAGATGGCCGAGT	TGACTCTTCAGCCATTCCCA	55.0	50.0	58.1	58.6	545-600 bp	1.0	78.9
trnS-GGA-rps4_p4	TGATAGGAGAGATGGCCGAG	TGACTCTTCAGCCATTCCCA	55.0	50.0	58.1	58.6	546-601 bp	1.0	78.9
trnS-GGA-rps4_p5	GATAGGAGAGATGGCCGAGT	GACTCTTCAGCCATTCCCAT	55.0	50.0	58.1	57.3	544-599 bp	1.0	78.2