Hotspot

trnE-UUC-trnT-GGU

LSC · 29616-30464 · 849 bp

Back to Frankenia

Pi 0.0118

MarkerSeek score 65.60

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 849
variable_sites: 10
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	936-940 bp	1.0	79.7
trnE-UUC-trnT-GGU_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	934-938 bp	1.0	79.7
trnE-UUC-trnT-GGU_p3	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	933-937 bp	1.0	79.2
trnE-UUC-trnT-GGU_p4	AGAGATGTCCTGAACCGCTA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.9	57.9	901-905 bp	1.0	79.0
trnE-UUC-trnT-GGU_p5	AGATGTCCTGAACCGCTAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.9	57.9	899-903 bp	1.0	79.0