primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GCGCTCAACCTACAGGAAC	20	19	50.0	57.9	58.2	58.5	20.3	0.0	1.711340	3856	4645	847-883	1.000000	70.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GCGCTCAACCTACAGGAAC	20	19	50.0	57.9	58.2	58.5	20.3	0.0	1.711340	3856	4645	846-882	1.000000	70.7
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TACCGTTGAGTTAGCAACCC	GCGCTCAACCTACAGGAAC	20	19	50.0	57.9	57.5	58.5	0.0	0.0	2.003677	3856	4645	833-869	1.000000	68.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCGCGGTCTCACAAACTTTA	GCGCTCAACCTACAGGAAC	20	19	45.0	57.9	57.5	58.5	6.1	0.0	2.055405	3856	4645	932-968	1.000000	67.5
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AGTCGCACTTAAAAGCCGAG	GCGCTCAACCTACAGGAAC	20	19	50.0	57.9	58.6	58.5	6.4	0.0	2.113457	3856	4645	858-894	1.000000	67.4
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ACGGAAAGAGAGGGATTCGA	AATCAAACCGAAAGACCCCT	20	20	50.0	45.0	58.2	57.0	0.0	0.0	1.123249	7409	8257	983-1014	1.000000	74.1
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	ACGGAAAGAGAGGGATTCGA	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	58.2	57.0	0.0	0.0	1.123249	7409	8257	982-1013	1.000000	74.1
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	TACGAATGACCCGTACAACG	AATCAAACCGAAAGACCCCT	20	20	50.0	45.0	57.7	57.0	0.0	0.0	1.232938	7409	8257	955-986	1.000000	73.3
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	TACGAATGACCCGTACAACG	ATCAAACCGAAAGACCCCTT	20	20	50.0	45.0	57.7	57.0	0.0	0.0	1.232938	7409	8257	954-985	1.000000	73.3
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	ACGGAAAGAGAGGGATTCGA	ACCGAAAGACCCCTTAACTC	20	20	50.0	50.0	58.2	56.9	0.0	0.0	1.296972	7409	8257	977-1008	1.000000	72.7
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	29616	30464	936-940	1.000000	79.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.151367	29616	30464	934-938	1.000000	79.7
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.217323	29616	30464	933-937	1.000000	79.2
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	AGAGATGTCCTGAACCGCTA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.248934	29616	30464	901-905	1.000000	79.0
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	AGATGTCCTGAACCGCTAGA	TACCGCTGAGTTAAAAGGGC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.248934	29616	30464	899-903	1.000000	79.0
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GCCCTTTTAACTCAGCGGTA	TCTCCGTAACCAGTCATCCA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.328919	30537	31960	1496-1571	1.000000	82.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	GGCGTAAGTCATCGGTTCAA	TCTCCGTAACCAGTCATCCA	20	20	50.0	50.0	58.3	57.8	0.0	0.0	0.508188	30537	31960	1459-1534	1.000000	80.6
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GCCCTTTTAACTCAGCGGTA	GGTCTCTCCGTAACCAGTCA	20	20	50.0	55.0	57.9	58.5	0.0	0.0	0.561045	30537	31960	1500-1575	1.000000	80.2
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GCCCTTTTAACTCAGCGGTA	AGCAATAAACCGGACCAACC	20	20	50.0	50.0	57.9	58.5	0.0	9.5	0.562158	30537	31960	1534-1609	1.000000	80.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GCCCTTTTAACTCAGCGGTA	GCAATAAACCGGACCAACCT	20	20	50.0	50.0	57.9	58.5	0.0	9.5	0.562158	30537	31960	1533-1608	1.000000	80.1
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	GATAGGAGAGATGGCCGAGT	TTGACTCTTCAGCCATTCCC	20	20	55.0	50.0	58.1	57.5	0.0	0.0	0.579026	44481	44873	546-601	1.000000	80.1
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	TGATAGGAGAGATGGCCGAG	TTGACTCTTCAGCCATTCCC	20	20	55.0	50.0	58.1	57.5	0.0	0.0	0.580393	44481	44873	547-602	1.000000	80.1
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	GATAGGAGAGATGGCCGAGT	TGACTCTTCAGCCATTCCCA	20	20	55.0	50.0	58.1	58.6	0.0	0.0	0.720924	44481	44873	545-600	1.000000	78.9
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	TGATAGGAGAGATGGCCGAG	TGACTCTTCAGCCATTCCCA	20	20	55.0	50.0	58.1	58.6	0.0	0.0	0.722290	44481	44873	546-601	1.000000	78.9
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	GATAGGAGAGATGGCCGAGT	GACTCTTCAGCCATTCCCAT	20	20	55.0	50.0	58.1	57.3	0.0	0.0	0.807349	44481	44873	544-599	1.000000	78.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TCCTGAAAGTAGAAATGCAA	CGGTTCAAGTCCGTATAGCC	20	20	35.0	55.0	52.4	58.2	0.0	0.0	5.764427	49603	50603	1111-1117	1.000000	41.3
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TCCTGAAAGTAGAAATGCAAA	CGGTTCAAGTCCGTATAGCC	21	20	33.3	55.0	53.3	58.2	0.0	0.0	5.814737	49603	50603	1111-1117	1.000000	41.3
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TCCTGAAAGTAGAAATGCAA	GGTTCAAGTCCGTATAGCCC	20	20	35.0	55.0	52.4	57.8	0.0	0.0	5.849369	49603	50603	1110-1116	1.000000	41.3
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCCTGAAAGTAGAAATGCAAA	GGTTCAAGTCCGTATAGCCC	21	20	33.3	55.0	53.3	57.8	0.0	0.0	5.899678	49603	50603	1110-1116	1.000000	41.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TCCTGAAAGTAGAAATGCAAAA	CGGTTCAAGTCCGTATAGCC	22	20	31.8	55.0	54.2	58.2	0.0	0.0	5.951314	49603	50603	1111-1117	1.000000	41.3
rpl33-rps18_p1	rpl33-rps18	1	ATCAAGCGGCATTTCGAGAT	GTTCCCCGGAGAATGAACTC	20	20	45.0	55.0	58.0	58.0	1.4	0.7	0.066547	66625	67240	1253-1254	1.000000	81.3
rpl33-rps18_p2	rpl33-rps18	2	GGTTCATGGCCAAGGGTAAA	GTTCCCCGGAGAATGAACTC	20	20	50.0	55.0	58.1	58.0	11.0	0.7	0.093953	66625	67240	1335-1336	1.000000	81.0
rpl33-rps18_p3	rpl33-rps18	3	AAAGGAGGTTCATGGCCAAG	GTTCCCCGGAGAATGAACTC	20	20	50.0	55.0	58.1	58.0	11.0	0.7	0.096720	66625	67240	1341-1342	1.000000	81.0
rpl33-rps18_p4	rpl33-rps18	4	AAGGTCGATGGAGGACAAGA	GTTCCCCGGAGAATGAACTC	20	20	50.0	55.0	58.1	58.0	0.0	0.7	0.087004	66625	67240	1518-1528	1.000000	80.9
rpl33-rps18_p5	rpl33-rps18	5	ATGCGTTGACATTCCCCTTT	GTTCCCCGGAGAATGAACTC	20	20	45.0	55.0	58.1	58.0	0.0	0.7	0.102233	66625	67240	1694-1705	1.000000	80.7
rpl16_p1	rpl16	1	CGATTGCTAGCGCCTATGAT	GTCGAGGGAATTGCACGTAT	20	20	50.0	50.0	58.0	58.1	10.8	13.2	0.065879	80439	81789	2170-2173	1.000000	80.3
rpl16_p2	rpl16	2	AAAACGCAAAACACCTGTGG	GTCGAGGGAATTGCACGTAT	20	20	45.0	50.0	58.0	58.1	1.9	13.2	0.067264	80439	81789	2028-2031	1.000000	80.3
rpl16_p3	rpl16	3	CGATTGCTAGCGCCTATGAT	TGCGCCTATACAGTTCGAAC	20	20	50.0	50.0	58.0	58.1	10.8	20.7	0.084539	80439	81789	1753-1756	1.000000	80.2
rpl16_p4	rpl16	4	TGTCCAAAACGCAAAACACC	GTCGAGGGAATTGCACGTAT	20	20	45.0	50.0	58.0	58.1	0.0	13.2	0.066398	80439	81789	2033-2033	0.500000	59.5
rpl16_p5	rpl16	5	CCAAAACGCAAAACACCTGT	GTCGAGGGAATTGCACGTAT	20	20	45.0	50.0	58.0	58.1	0.0	13.2	0.067264	80439	81789	2030-2030	0.500000	59.5
trnR-ACG-trnN-GUU_p1	trnR-ACG-trnN-GUU	1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	20	20	55.0	55.0	57.8	58.0	18.9	0.0	0.218238	107553	108013	602-653	1.000000	79.3
trnR-ACG-trnN-GUU_p2	trnR-ACG-trnN-GUU	2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	20	20	55.0	50.0	57.8	58.2	18.9	18.9	0.372539	107553	108013	553-604	1.000000	78.1
trnR-ACG-trnN-GUU_p3	trnR-ACG-trnN-GUU	3	CAGAGGATTAGAGCACGTGG	TTCGAATCCTACCTGGGGAG	20	20	55.0	55.0	57.8	58.2	18.9	0.0	0.426384	107553	108013	543-594	1.000000	77.7
trnR-ACG-trnN-GUU_p4	trnR-ACG-trnN-GUU	4	CAGAGGATTAGAGCACGTGG	CCAATCTCCCGTTGTTCCTC	20	20	55.0	55.0	57.8	58.3	18.9	0.0	0.476840	107553	108013	610-661	1.000000	77.2
trnR-ACG-trnN-GUU_p5	trnR-ACG-trnN-GUU	5	CAGAGGATTAGAGCACGTGG	CGATTGGTCGTAGGTTCGAA	20	20	55.0	50.0	57.8	57.7	18.9	0.0	0.483095	107553	108013	557-608	1.000000	77.2
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	ATCTCCCCAGGTAGGATTCG	TCCGTTCAACCAACAAGGAA	20	20	55.0	45.0	58.0	57.6	0.0	0.0	0.438656	108086	109536	2063-2121	1.000000	78.0
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	ATCTCCCCAGGTAGGATTCG	TTCCGTTCAACCAACAAGGA	20	20	55.0	45.0	58.0	57.6	0.0	0.0	0.438656	108086	109536	2064-2122	1.000000	78.0
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	ATCTCACTTCACACCTCGGA	TCCGTTCAACCAACAAGGAA	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.497020	108086	109536	2737-2846	1.000000	77.4
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	ATCTCACTTCACACCTCGGA	TTCCGTTCAACCAACAAGGA	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.497020	108086	109536	2738-2847	1.000000	77.4
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	TCTTAACCCGGAAATGGCTG	TCCGTTCAACCAACAAGGAA	20	20	50.0	45.0	57.9	57.6	0.7	0.0	0.544534	108086	109536	2678-2787	1.000000	77.0
ndhF-rpl32_p1	ndhF-rpl32	1	AATCCAAAGTCTCCGACACG	TCGCTTTCGAAAGAGATGCT	20	20	50.0	45.0	57.9	57.6	0.0	35.9	0.450567	111766	112737	1662-1679	1.000000	77.4
ndhF-rpl32_p2	ndhF-rpl32	2	CCAAAGTCTCCGACACGATT	TCGCTTTCGAAAGAGATGCT	20	20	50.0	45.0	57.9	57.6	0.0	35.9	0.450567	111766	112737	1659-1676	1.000000	77.4
ndhF-rpl32_p3	ndhF-rpl32	3	ACCACACATGAAGCGGAAAT	TCGCTTTCGAAAGAGATGCT	20	20	45.0	45.0	58.1	57.6	0.0	35.9	0.460581	111766	112737	1844-1861	1.000000	77.3
ndhF-rpl32_p4	ndhF-rpl32	4	AAAAGACCACATACGCCGAA	TCGCTTTCGAAAGAGATGCT	20	20	45.0	45.0	57.8	57.6	0.0	35.9	0.543132	111766	112737	1222-1239	1.000000	76.8
ndhF-rpl32_p5	ndhF-rpl32	5	AAAGACCACATACGCCGAAA	TCGCTTTCGAAAGAGATGCT	20	20	45.0	45.0	57.8	57.6	0.0	35.9	0.543132	111766	112737	1221-1238	1.000000	76.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGCATCTCTTTCGAAAGCGA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.6	57.9	27.4	0.0	0.494558	112891	114030	1228-1296	1.000000	78.0
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGCATCTCTTTCGAAAGCGA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.6	57.8	27.4	0.0	0.555285	112891	114030	1221-1289	1.000000	77.5
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGCATCTCTTTCGAAAGCGA	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.6	57.8	27.4	0.0	0.566576	112891	114030	1227-1295	1.000000	77.4
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGCATCTCTTTCGAAAGCGA	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.6	57.8	27.4	0.0	0.566576	112891	114030	1226-1294	1.000000	77.4
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGCATCTCTTTCGAAAGCGA	GAGCAGCGTGTCTACCAATT	20	20	45.0	50.0	57.6	58.3	27.4	0.0	0.643428	112891	114030	1222-1290	1.000000	76.8
trnL-UAG-ccsA_p1	trnL-UAG-ccsA	1	TAGACACGCTGCTCTTAGGA	ACAATCATTGAAAAAGAAATCTGAGT	20	26	50.0	26.9	57.9	56.4	0.0	3.8	7.763289	114111	114233	239-240	1.000000	42.0
trnL-UAG-ccsA_p2	trnL-UAG-ccsA	2	GTAGACACGCTGCTCTTAGG	ACAATCATTGAAAAAGAAATCTGAGT	20	26	55.0	26.9	57.8	56.4	0.0	3.8	7.835307	114111	114233	240-241	1.000000	42.0
trnL-UAG-ccsA_p3	trnL-UAG-ccsA	3	AAATTGGTAGACACGCTGCT	ACAATCATTGAAAAAGAAATCTGAGT	20	26	45.0	26.9	57.8	56.4	0.0	3.8	7.824016	114111	114233	246-247	1.000000	41.9
trnL-UAG-ccsA_p4	trnL-UAG-ccsA	4	AACAAGCCGCTATGGTGAAA	ACAATCATTGAAAAAGAAATCTGAGT	20	26	45.0	26.9	58.1	56.4	0.0	3.8	7.734725	114111	114233	263-264	1.000000	41.8
trnL-UAG-ccsA_p5	trnL-UAG-ccsA	5	ACAAGCCGCTATGGTGAAAT	ACAATCATTGAAAAAGAAATCTGAGT	20	26	45.0	26.9	57.9	56.4	0.0	3.8	7.767783	114111	114233	262-263	1.000000	41.8
ndhD-psaC_p1	ndhD-psaC	1	GAAAACGAAAGAACCTGCGA	AGAGATGTGAATCTGCCTGC	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.359460	116914	117049	290-291	1.000000	71.1
ndhD-psaC_p2	ndhD-psaC	2	AGAAAACGAAAGAACCTGCG	AGAGATGTGAATCTGCCTGC	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.367554	116914	117049	291-292	1.000000	71.0
ndhD-psaC_p3	ndhD-psaC	3	GAAAACGAAAGAACCTGCGA	CAGAGGACTGTGTTGGTTGT	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.362704	116914	117049	311-312	1.000000	70.9
ndhD-psaC_p4	ndhD-psaC	4	AGAAAACGAAAGAACCTGCG	CAGAGGACTGTGTTGGTTGT	20	20	45.0	50.0	57.0	57.7	0.0	0.0	1.370798	116914	117049	312-313	1.000000	70.8
ndhD-psaC_p5	ndhD-psaC	5	GAAAACGAAAGAACCTGCGAA	AGAGATGTGAATCTGCCTGC	21	20	42.9	50.0	57.7	57.7	0.0	0.0	1.657056	116914	117049	290-291	1.000000	68.7
ndhG-ndhI_p1	ndhG-ndhI	1	TCCCAAAGAAAAGGCAGCAA	TCAAATTGCTTTGGGTCGTT	20	20	45.0	40.0	58.2	56.4	0.0	0.0	1.770433	118556	118863	523-528	1.000000	70.7
ndhG-ndhI_p2	ndhG-ndhI	2	TCCCAAAGAAAAGGCAGCAA	ATCAAATTGCTTTGGGTCGT	20	20	45.0	40.0	58.2	56.2	0.0	0.0	2.001405	118556	118863	524-529	1.000000	68.9
ndhG-ndhI_p3	ndhG-ndhI	3	TCCCAAAGAAAAGGCAGCAA	TCAAATTGCTTTGGGTCGTTT	20	21	45.0	38.1	58.2	57.2	0.0	0.0	2.023373	118556	118863	523-528	1.000000	68.7
ndhG-ndhI_p4	ndhG-ndhI	4	CCCAAAGAAAAGGCAGCAAA	TCAAATTGCTTTGGGTCGTT	20	20	45.0	40.0	57.4	56.4	0.0	0.0	2.175812	118556	118863	522-527	1.000000	67.5
ndhG-ndhI_p5	ndhG-ndhI	5	TCCCAAAGAAAAGGCAGCAA	ATCAAATTGCTTTGGGTCGTT	20	21	45.0	38.1	58.2	57.0	0.0	0.0	2.240682	118556	118863	524-529	1.000000	66.9
ycf1_p1	ycf1	1	ACGGATTAATCGAGCAGTTCT	GAATTTCCTTTTGGCTCGCC	21	20	42.9	50.0	57.2	58.0	9.3	0.0	1.806657	125910	126029	525-525	1.000000	67.7
ycf1_p2	ycf1	2	AGGCTTGTGAGAGAAAGGAT	GAATTTCCTTTTGGCTCGCC	20	20	45.0	50.0	56.1	58.0	0.0	0.0	1.868259	125910	126029	826-826	1.000000	66.7
ycf1_p3	ycf1	3	ACGGATTAATCGAGCAGTTCT	AAAGGCGTGGAATCGAGAAA	21	20	42.9	45.0	57.2	57.8	9.3	0.0	1.981860	125910	126029	757-757	1.000000	65.8
ycf1_p4	ycf1	4	AGGCTTGTGAGAGAAAGGAT	AAAGGCGTGGAATCGAGAAA	20	20	45.0	45.0	56.1	57.8	0.0	0.0	2.043463	125910	126029	1058-1058	1.000000	65.1
ycf1_p5	ycf1	5	ACGGATTAATCGAGCAGTTCT	CGAAATTGCAGAAACGTGGG	21	20	42.9	50.0	57.2	58.3	9.3	0.0	2.117646	125910	126029	925-925	1.000000	65.0
trnN-GUU-trnR-ACG_p1	trnN-GUU-trnR-ACG	1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.0	57.8	0.0	18.9	0.218238	129331	129791	602-653	1.000000	79.3
trnN-GUU-trnR-ACG_p2	trnN-GUU-trnR-ACG	2	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	58.2	57.8	18.9	18.9	0.372539	129331	129791	553-604	1.000000	78.1
trnN-GUU-trnR-ACG_p3	trnN-GUU-trnR-ACG	3	TTCGAATCCTACCTGGGGAG	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.2	57.8	0.0	18.9	0.426384	129331	129791	543-594	1.000000	77.7
trnN-GUU-trnR-ACG_p4	trnN-GUU-trnR-ACG	4	CCAATCTCCCGTTGTTCCTC	CAGAGGATTAGAGCACGTGG	20	20	55.0	55.0	58.3	57.8	0.0	18.9	0.476840	129331	129791	610-661	1.000000	77.2
trnN-GUU-trnR-ACG_p5	trnN-GUU-trnR-ACG	5	CGATTGGTCGTAGGTTCGAA	CAGAGGATTAGAGCACGTGG	20	20	50.0	55.0	57.7	57.8	0.0	18.9	0.483095	129331	129791	557-608	1.000000	77.2