Hotspot

rps2

LSC · 16765-17475 · 711 bp

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Pi 0

MarkerSeek score 36.70

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	TCTCTTTTGTGCGATTCAGA	CGGTAGAAGAGAAGGTTCCG	40.0	55.0	55.0	57.4	902 bp	1.0	51.7
rps2_p2	TCTCTTTTGTGCGATTCAGA	AAGAGAAGGTTCCGTGGGAA	40.0	50.0	55.0	58.6	896 bp	1.0	51.7
rps2_p3	TGCGATTCAGAAATAGTAACCGA	CGGTAGAAGAGAAGGTTCCG	39.1	55.0	58.0	57.4	893 bp	1.0	51.5
rps2_p4	TGCGATTCAGAAATAGTAACCGA	AAGAGAAGGTTCCGTGGGAA	39.1	50.0	58.0	58.6	887 bp	1.0	51.4
rps2_p5	GTGCGATTCAGAAATAGTAACCG	CGGTAGAAGAGAAGGTTCCG	43.5	55.0	57.9	57.4	894 bp	1.0	51.0