Hotspot

rps16

LSC · 5607-6754 · 1148 bp

Back to Frangula

Pi 0.000871

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1148
variable_sites: 1
pi: 0.000871
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	GCGGGGAATAGCAGAGAAAA	CGGCCGAACCAATGACTATT	50.0	50.0	58.0	58.3	1324 bp	1.0	77.1
rps16_p2	GCGGGGAATAGCAGAGAAAA	TATTATGTACCGGCCGAACC	50.0	50.0	58.0	57.2	1334 bp	1.0	73.2
rps16_p3	GCGGGGAATAGCAGAGAAAA	GCCGAACCAATGACTATTCG	50.0	50.0	58.0	57.0	1322 bp	1.0	71.6
rps16_p4	GCGGGGAATAGCAGAGAAAA	TTATTATGTACCGGCCGAACC	50.0	47.6	58.0	57.9	1335 bp	1.0	70.9
rps16_p5	GCGGGGAATAGCAGAGAAAA	TATGTACCGGCCGAACCAAT	50.0	50.0	58.0	59.2	1331 bp	1.0	70.4