Hotspot

matK

LSC · 2284-3795 · 1512 bp

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Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK

Feature Metadata

label_name: matK
feature_type: gene
region: LSC
length_bp: 1512
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK_p1	CATTTCCGTAGTCGCACAAC	AAACCCCGTTCAAGTCGAAT	50.0	45.0	57.5	57.7	1560 bp	1.0	73.8
matK_p2	CATTTCCGTAGTCGCACAAC	AACCCCGTTCAAGTCGAATT	50.0	45.0	57.5	57.7	1559 bp	1.0	73.8
matK_p3	CATTTCCGTAGTCGCACAAC	ACCCCGTTCAAGTCGAATTT	50.0	45.0	57.5	57.7	1558 bp	1.0	73.8
matK_p4	ATTTCCGTAGTCGCACAACC	AACCCCGTTCAAGTCGAATT	50.0	45.0	58.6	57.7	1558 bp	1.0	73.4
matK_p5	ATTTCCGTAGTCGCACAACC	ACCCCGTTCAAGTCGAATTT	50.0	45.0	58.6	57.7	1557 bp	1.0	73.4