Hotspot

ccsA

SSC · 120619-121584 · 966 bp

Back to Frangula

Pi 0
MarkerSeek score 40.00
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
966
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TGTAATTGAAAGACCTTCCTTGT TTGTCAAGCCCGTTCTTCAA 34.8 45.0 56.0 58.0 1034 bp 1.0 40.0
ccsA_p2 TGTAATTGAAAGACCTTCCTTGT TTTGTCAAGCCCGTTCTTCA 34.8 45.0 56.0 58.0 1035 bp 1.0 40.0
ccsA_p3 TGTAATTGAAAGACCTTCCTTGT ATTCACGGGGTTCTCAATGG 34.8 50.0 56.0 57.9 1106 bp 1.0 40.0
ccsA_p4 TGTAATTGAAAGACCTTCCTTGT GTGATTCACGGGGTTCTCAA 34.8 50.0 56.0 57.8 1109 bp 1.0 40.0
ccsA_p5 TGTAATTGAAAGACCTTCCTTGT TGAAGTGATTCACGGGGTTC 34.8 50.0 56.0 57.8 1113 bp 1.0 40.0