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ccsA-ndhD

SSC · 121585-121860 · 276 bp

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Pi 0.00362

MarkerSeek score 54.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 276
variable_sites: 1
pi: 0.00362
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TAAATTCCGCAATTGTGGCC	TCCGGATTTCGTTTTCTCACT	45.0	42.9	57.3	57.6	474 bp	1.0	63.7
ccsA-ndhD_p2	AAATTCCGCAATTGTGGCCT	TCCGGATTTCGTTTTCTCACT	45.0	42.9	59.0	57.6	473 bp	1.0	60.8
ccsA-ndhD_p3	TAAATTCCGCAATTGTGGCC	TCAGTTGAGAAGGTTGAAGCT	45.0	42.9	57.3	57.2	452 bp	1.0	60.1
ccsA-ndhD_p4	GGGTTCCACAGTTATGGTTCA	TCCGGATTTCGTTTTCTCACT	47.6	42.9	57.9	57.6	397 bp	1.0	59.8
ccsA-ndhD_p5	TAAATTCCGCAATTGTGGCC	TCCGGATTTCGTTTTCTCAC	45.0	45.0	57.3	56.1	474 bp	1.0	59.6