Hotspot

rpl32-trnL-UAG

SSC · 119075-120406 · 1332 bp

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Pi 0
MarkerSeek score 59.20
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1332
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGAAGGGATATTGGGTAGCA AGCGTGTCTACCGATTTCAC 47.6 50.0 57.9 58.0 1451-1457 bp 1.0 71.7
rpl32-trnL-UAG_p2 AGGAAGGGATATTGGGTAGCA CAGCGTGTCTACCGATTTCA 47.6 50.0 57.9 58.0 1452-1458 bp 1.0 71.7
rpl32-trnL-UAG_p3 AGGAAGGGATATTGGGTAGCA TCCTAAGAGCAGCGTGTCTA 47.6 50.0 57.9 57.9 1461-1467 bp 1.0 70.7
rpl32-trnL-UAG_p4 GCGAAGTCTCTTTCTACGAGG AGCGTGTCTACCGATTTCAC 52.4 50.0 58.2 58.0 1412-1418 bp 1.0 70.6
rpl32-trnL-UAG_p5 GCGAAGTCTCTTTCTACGAGG CAGCGTGTCTACCGATTTCA 52.4 50.0 58.2 58.0 1413-1419 bp 1.0 70.6