Hotspot

petA-psbJ

LSC · 67096-68163 · 1068 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1068
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGTGTCCAAGGACTTTTGCT TCTTGTGATCGGTTTAATCGGT 50.0 40.9 58.7 57.8 1246 bp 1.0 56.9
petA-psbJ_p2 TCCATTACGTGTCCAAGGAC TCTTGTGATCGGTTTAATCGGT 50.0 40.9 57.2 57.8 1253 bp 1.0 56.4
petA-psbJ_p3 CGTGTCCAAGGACTTTTGCT ACTGGTATTCTTGTGATCGGT 50.0 42.9 58.7 56.7 1254 bp 1.0 56.4
petA-psbJ_p4 CCATTACGTGTCCAAGGACT TCTTGTGATCGGTTTAATCGGT 50.0 40.9 57.2 57.8 1252 bp 1.0 56.3
petA-psbJ_p5 TCCATTACGTGTCCAAGGAC ACTGGTATTCTTGTGATCGGT 50.0 42.9 57.2 56.7 1261 bp 1.0 55.9