Hotspot

trnS-GCU-trnG-UCC

LSC · 8835-9689 · 855 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
855
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAAAGTGTCGACGAAAACGG TTAATCAAACCGAGGGACCC 50.0 50.0 58.0 57.2 1039 bp 1.0 73.7
trnS-GCU-trnG-UCC_p2 CAATCCGACGCTTTAGTCCA TTAATCAAACCGAGGGACCC 50.0 50.0 58.0 57.2 969 bp 1.0 73.6
trnS-GCU-trnG-UCC_p3 AATCCGACGCTTTAGTCCAC TTAATCAAACCGAGGGACCC 50.0 50.0 58.0 57.2 968 bp 1.0 73.6
trnS-GCU-trnG-UCC_p4 AAGTGTCGACGAAAACGGAA TTAATCAAACCGAGGGACCC 45.0 50.0 58.1 57.2 1037 bp 1.0 73.1
trnS-GCU-trnG-UCC_p5 AGTGTCGACGAAAACGGAAA TTAATCAAACCGAGGGACCC 45.0 50.0 58.1 57.2 1036 bp 1.0 73.1