Hotspot

trnH-GUG-psbA

LSC · 286-642 · 357 bp

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Pi 0

MarkerSeek score 34.90

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCGAGCTAACTTGGGTATGG	55.0	55.0	58.1	57.8	476 bp	1.0	77.0
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCGAGCTAACTTGGGTATGG	50.0	55.0	57.9	57.8	490 bp	1.0	77.0
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCGAGCTAACTTGGGTATGG	50.0	55.0	57.9	57.8	489 bp	1.0	77.0
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CCGAGCTAACTTGGGTATGG	50.0	55.0	57.9	57.8	491 bp	1.0	77.0
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	ACTTCCCTCTAGACCTAGCG	55.0	55.0	58.1	57.6	429 bp	1.0	76.2