primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCGAGCTAACTTGGGTATGG	20	20	55.0	55.0	58.1	57.8	0.0	0.0	0.369413	286	642	476-476	1.000000	77.0
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCGAGCTAACTTGGGTATGG	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.373775	286	642	490-490	1.000000	77.0
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCGAGCTAACTTGGGTATGG	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.375282	286	642	489-489	1.000000	77.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCGAGCTAACTTGGGTATGG	20	20	50.0	55.0	57.9	57.8	0.0	0.0	0.375282	286	642	491-491	1.000000	77.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	ACTTCCCTCTAGACCTAGCG	20	20	55.0	55.0	58.1	57.6	0.0	0.0	0.480626	286	642	429-429	1.000000	76.2
psbA_p1	psbA	1	ACACTAATACCAAAGCGAAGTC	TTATGTGCTTGGGAGTCCCT	22	20	40.9	50.0	56.5	58.3	0.0	28.7	3.864656	643	1704	1138-1138	1.000000	49.1
psbA_p2	psbA	2	ACACTAATACCAAAGCGAAGTC	TTTATGTGCTTGGGAGTCCC	22	20	40.9	50.0	56.5	57.5	0.0	22.5	4.040889	643	1704	1139-1139	1.000000	47.7
psbA_p3	psbA	3	ACACTAATACCAAAGCGAAGT	TTATGTGCTTGGGAGTCCCT	21	20	38.1	50.0	55.3	58.3	0.0	28.7	4.079140	643	1704	1138-1138	1.000000	47.4
psbA_p4	psbA	4	ACACTAATACCAAAGCGAAGT	TTTATGTGCTTGGGAGTCCC	21	20	38.1	50.0	55.3	57.5	0.0	22.5	4.255373	643	1704	1139-1139	1.000000	46.0
psbA_p5	psbA	5	ACACTAATACCAAAGCGAAGTC	ATTTATGTGCTTGGGAGTCCC	22	21	40.9	47.6	56.5	58.0	0.0	22.5	4.551905	643	1704	1140-1140	1.000000	43.6
matK_p1	matK	1	CATTTCCGTAGTCGCACAAC	AAACCCCGTTCAAGTCGAAT	20	20	50.0	45.0	57.5	57.7	0.0	0.0	0.769532	2284	3795	1560-1560	1.000000	73.8
matK_p2	matK	2	CATTTCCGTAGTCGCACAAC	AACCCCGTTCAAGTCGAATT	20	20	50.0	45.0	57.5	57.7	0.0	0.0	0.769532	2284	3795	1559-1559	1.000000	73.8
matK_p3	matK	3	CATTTCCGTAGTCGCACAAC	ACCCCGTTCAAGTCGAATTT	20	20	50.0	45.0	57.5	57.7	0.0	0.0	0.769532	2284	3795	1558-1558	1.000000	73.8
matK_p4	matK	4	ATTTCCGTAGTCGCACAACC	AACCCCGTTCAAGTCGAATT	20	20	50.0	45.0	58.6	57.7	0.0	0.0	0.830012	2284	3795	1558-1558	1.000000	73.4
matK_p5	matK	5	ATTTCCGTAGTCGCACAACC	ACCCCGTTCAAGTCGAATTT	20	20	50.0	45.0	58.6	57.7	0.0	0.0	0.830012	2284	3795	1557-1557	1.000000	73.4
matK-trnK-UUU_p1	matK-trnK-UUU	1	GAGGTCATGCTGCTGAGATC	AACGGTAGAGTACTCGGCTT	20	20	55.0	50.0	58.1	58.2	0.0	17.1	0.299951	3796	4536	819-819	1.000000	77.6
matK-trnK-UUU_p2	matK-trnK-UUU	2	GAGGTCATGCTGCTGAGATC	ACGGTAGAGTACTCGGCTTT	20	20	55.0	50.0	58.1	58.2	0.0	17.1	0.299951	3796	4536	818-818	1.000000	77.6
matK-trnK-UUU_p3	matK-trnK-UUU	3	ATAGGAGGTCATGCTGCTGA	AACGGTAGAGTACTCGGCTT	20	20	50.0	50.0	58.2	58.2	0.0	17.1	0.369794	3796	4536	823-823	1.000000	77.0
matK-trnK-UUU_p4	matK-trnK-UUU	4	ATAGGAGGTCATGCTGCTGA	ACGGTAGAGTACTCGGCTTT	20	20	50.0	50.0	58.2	58.2	0.0	17.1	0.369794	3796	4536	822-822	1.000000	77.0
matK-trnK-UUU_p5	matK-trnK-UUU	5	GAGGTCATGCTGCTGAGATC	GGGTTGCTAACTCAACGGTA	20	20	55.0	50.0	58.1	57.5	0.0	0.0	0.592288	3796	4536	832-832	1.000000	75.3
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AGTCGCGGTCTTACAAACTC	ATTTCAAAGAAAGCGGGGGT	20	20	50.0	45.0	57.9	58.0	6.1	0.0	0.071421	4574	5606	1213-1213	1.000000	79.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	GTCGCGGTCTTACAAACTCT	ATTTCAAAGAAAGCGGGGGT	20	20	50.0	45.0	57.9	58.0	6.1	0.0	0.071421	4574	5606	1212-1212	1.000000	79.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AGTCGCGGTCTTACAAACTC	TCCTGCAAAAAGGAGCTCAG	20	20	50.0	50.0	57.9	58.1	6.1	17.2	0.160149	4574	5606	1254-1254	1.000000	78.7
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	GTCGCGGTCTTACAAACTCT	TCCTGCAAAAAGGAGCTCAG	20	20	50.0	50.0	57.9	58.1	6.1	17.2	0.160149	4574	5606	1253-1253	1.000000	78.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	ATTTCAAAGAAAGCGGGGGT	20	20	50.0	45.0	58.2	58.0	20.3	0.0	0.178101	4574	5606	1129-1129	1.000000	78.6
rps16_p1	rps16	1	GCGGGGAATAGCAGAGAAAA	CGGCCGAACCAATGACTATT	20	20	50.0	50.0	58.0	58.3	0.0	24.1	0.374692	5607	6754	1324-1324	1.000000	77.1
rps16_p2	rps16	2	GCGGGGAATAGCAGAGAAAA	TATTATGTACCGGCCGAACC	20	20	50.0	50.0	58.0	57.2	0.0	30.7	0.856517	5607	6754	1334-1334	1.000000	73.2
rps16_p3	rps16	3	GCGGGGAATAGCAGAGAAAA	GCCGAACCAATGACTATTCG	20	20	50.0	50.0	58.0	57.0	0.0	0.0	1.056963	5607	6754	1322-1322	1.000000	71.6
rps16_p4	rps16	4	GCGGGGAATAGCAGAGAAAA	TTATTATGTACCGGCCGAACC	20	21	50.0	47.6	58.0	57.9	0.0	30.7	1.148834	5607	6754	1335-1335	1.000000	70.9
rps16_p5	rps16	5	GCGGGGAATAGCAGAGAAAA	TATGTACCGGCCGAACCAAT	20	20	50.0	50.0	58.0	59.2	0.0	30.7	1.206421	5607	6754	1331-1331	1.000000	70.4
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAAAGTGTCGACGAAAACGG	TTAATCAAACCGAGGGACCC	20	20	50.0	50.0	58.0	57.2	11.6	0.6	0.793465	8835	9689	1039-1039	1.000000	73.7
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	CAATCCGACGCTTTAGTCCA	TTAATCAAACCGAGGGACCC	20	20	50.0	50.0	58.0	57.2	0.0	0.6	0.797870	8835	9689	969-969	1.000000	73.6
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AATCCGACGCTTTAGTCCAC	TTAATCAAACCGAGGGACCC	20	20	50.0	50.0	58.0	57.2	0.0	0.6	0.799258	8835	9689	968-968	1.000000	73.6
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AAGTGTCGACGAAAACGGAA	TTAATCAAACCGAGGGACCC	20	20	45.0	50.0	58.1	57.2	11.6	0.6	0.868059	8835	9689	1037-1037	1.000000	73.1
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGTGTCGACGAAAACGGAAA	TTAATCAAACCGAGGGACCC	20	20	45.0	50.0	58.1	57.2	11.6	0.6	0.868059	8835	9689	1036-1036	1.000000	73.1
rps2_p1	rps2	1	TCTCTTTTGTGCGATTCAGA	CGGTAGAAGAGAAGGTTCCG	20	20	40.0	55.0	55.0	57.4	0.0	0.0	3.535687	16765	17475	902-902	1.000000	51.7
rps2_p2	rps2	2	TCTCTTTTGTGCGATTCAGA	AAGAGAAGGTTCCGTGGGAA	20	20	40.0	50.0	55.0	58.6	0.0	0.0	3.541224	16765	17475	896-896	1.000000	51.7
rps2_p3	rps2	3	TGCGATTCAGAAATAGTAACCGA	CGGTAGAAGAGAAGGTTCCG	23	20	39.1	55.0	58.0	57.4	0.0	0.0	3.564708	16765	17475	893-893	1.000000	51.5
rps2_p4	rps2	4	TGCGATTCAGAAATAGTAACCGA	AAGAGAAGGTTCCGTGGGAA	23	20	39.1	50.0	58.0	58.6	0.0	0.0	3.570245	16765	17475	887-887	1.000000	51.4
rps2_p5	rps2	5	GTGCGATTCAGAAATAGTAACCG	CGGTAGAAGAGAAGGTTCCG	23	20	43.5	55.0	57.9	57.4	0.0	0.0	3.630492	16765	17475	894-894	1.000000	51.0
rps2-rpoC2_p1	rps2-rpoC2	1	GGTGCCATTCTAGGATTCCA	ATTCGAGCGGGAAATGAGAG	20	20	50.0	50.0	57.0	57.8	5.3	0.0	1.260852	17476	17678	419-419	1.000000	70.1
rps2-rpoC2_p2	rps2-rpoC2	2	CCTGCTTCCATCATCTCTTCC	ATTCGAGCGGGAAATGAGAG	21	20	52.4	50.0	58.2	57.8	0.0	0.0	1.382524	17476	17678	371-371	1.000000	69.2
rps2-rpoC2_p3	rps2-rpoC2	3	TGACCAAAATGAACTCCTGCT	ATTCGAGCGGGAAATGAGAG	21	20	42.9	50.0	57.8	57.8	0.0	0.0	1.459403	17476	17678	386-386	1.000000	68.5
rps2-rpoC2_p4	rps2-rpoC2	4	ACTCCTGCTTCCATCATCTCT	ATTCGAGCGGGAAATGAGAG	21	20	47.6	50.0	58.2	57.8	0.0	0.0	1.469405	17476	17678	374-374	1.000000	68.5
rps2-rpoC2_p5	rps2-rpoC2	5	TGAACTCCTGCTTCCATCATC	ATTCGAGCGGGAAATGAGAG	21	20	47.6	50.0	57.7	57.8	0.0	0.0	1.503789	17476	17678	377-377	1.000000	68.2
petA-psbJ_p1	petA-psbJ	1	CGTGTCCAAGGACTTTTGCT	TCTTGTGATCGGTTTAATCGGT	20	22	50.0	40.9	58.7	57.8	19.2	0.0	2.887628	67096	68163	1246-1246	1.000000	56.9
petA-psbJ_p2	petA-psbJ	2	TCCATTACGTGTCCAAGGAC	TCTTGTGATCGGTTTAATCGGT	20	22	50.0	40.9	57.2	57.8	12.3	0.0	2.951572	67096	68163	1253-1253	1.000000	56.4
petA-psbJ_p3	petA-psbJ	3	CGTGTCCAAGGACTTTTGCT	ACTGGTATTCTTGTGATCGGT	20	21	50.0	42.9	58.7	56.7	19.2	0.0	2.952521	67096	68163	1254-1254	1.000000	56.4
petA-psbJ_p4	petA-psbJ	4	CCATTACGTGTCCAAGGACT	TCTTGTGATCGGTTTAATCGGT	20	22	50.0	40.9	57.2	57.8	19.2	0.0	2.959161	67096	68163	1252-1252	1.000000	56.3
petA-psbJ_p5	petA-psbJ	5	TCCATTACGTGTCCAAGGAC	ACTGGTATTCTTGTGATCGGT	20	21	50.0	42.9	57.2	56.7	12.3	0.0	3.016465	67096	68163	1261-1261	1.000000	55.9
psbL_p1	psbL	1	ATTTCATCAGGGGGTCATGC	CTGGCTGTACCTACCGTTTC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.083658	68426	68542	322-322	1.000000	79.6
psbL_p2	psbL	2	TTCATCAGGGGGTCATGCTA	CTGGCTGTACCTACCGTTTC	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.114038	68426	68542	320-320	1.000000	79.3
psbL_p3	psbL	3	ATTTCATCAGGGGGTCATGC	ATGGTTAGCTGTTCACGGAC	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.172450	68426	68542	341-341	1.000000	78.9
psbL_p4	psbL	4	TTCATCAGGGGGTCATGCTA	ATGGTTAGCTGTTCACGGAC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.202829	68426	68542	339-339	1.000000	78.6
psbL_p5	psbL	5	ATCAGGGGGTCATGCTAGAG	CTGGCTGTACCTACCGTTTC	20	20	55.0	55.0	58.3	58.0	0.0	0.0	0.278908	68426	68542	317-317	1.000000	78.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	GCGGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.0	6.4	15.6	0.051764	68946	70328	1528-1528	1.000000	79.6
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	AAGCGGCTAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.0	6.4	1.4	0.057305	68946	70328	1530-1530	1.000000	79.6
psbE-petL_p3	psbE-petL	3	AGAACGTTCTCCTGTGCTTC	GCGGCTAGTAGAAAACCGAA	20	20	50.0	50.0	57.8	58.0	24.9	15.6	0.156772	68946	70328	1451-1451	1.000000	78.8
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	AAGCGGCTAGTAGAAAACCG	20	20	50.0	50.0	57.8	58.0	24.9	1.4	0.162314	68946	70328	1453-1453	1.000000	78.8
psbE-petL_p5	psbE-petL	5	ACGAATAACCAACCCGCAAT	GCGGCTAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.0	0.0	15.6	0.171450	68946	70328	1537-1537	1.000000	78.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGAAGGGATATTGGGTAGCA	AGCGTGTCTACCGATTTCAC	21	20	47.6	50.0	57.9	58.0	0.0	0.0	1.082688	119075	120406	1451-1457	1.000000	71.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGAAGGGATATTGGGTAGCA	CAGCGTGTCTACCGATTTCA	21	20	47.6	50.0	57.9	58.0	0.0	0.0	1.084058	119075	120406	1452-1458	1.000000	71.7
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGAAGGGATATTGGGTAGCA	TCCTAAGAGCAGCGTGTCTA	21	20	47.6	50.0	57.9	57.9	0.0	0.0	1.199135	119075	120406	1461-1467	1.000000	70.7
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GCGAAGTCTCTTTCTACGAGG	AGCGTGTCTACCGATTTCAC	21	20	52.4	50.0	58.2	58.0	0.0	0.0	1.219800	119075	120406	1412-1418	1.000000	70.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GCGAAGTCTCTTTCTACGAGG	CAGCGTGTCTACCGATTTCA	21	20	52.4	50.0	58.2	58.0	0.0	0.0	1.221171	119075	120406	1413-1419	1.000000	70.6
ccsA_p1	ccsA	1	TGTAATTGAAAGACCTTCCTTGT	TTGTCAAGCCCGTTCTTCAA	23	20	34.8	45.0	56.0	58.0	0.0	0.0	5.013218	120619	121584	1034-1034	1.000000	40.0
ccsA_p2	ccsA	2	TGTAATTGAAAGACCTTCCTTGT	TTTGTCAAGCCCGTTCTTCA	23	20	34.8	45.0	56.0	58.0	0.0	0.0	5.013218	120619	121584	1035-1035	1.000000	40.0
ccsA_p3	ccsA	3	TGTAATTGAAAGACCTTCCTTGT	ATTCACGGGGTTCTCAATGG	23	20	34.8	50.0	56.0	57.9	0.0	0.0	5.096427	120619	121584	1106-1106	1.000000	40.0
ccsA_p4	ccsA	4	TGTAATTGAAAGACCTTCCTTGT	GTGATTCACGGGGTTCTCAA	23	20	34.8	50.0	56.0	57.8	0.0	0.1	5.146569	120619	121584	1109-1109	1.000000	40.0
ccsA_p5	ccsA	5	TGTAATTGAAAGACCTTCCTTGT	TGAAGTGATTCACGGGGTTC	23	20	34.8	50.0	56.0	57.8	0.0	11.0	5.146569	120619	121584	1113-1113	1.000000	40.0
ccsA-ndhD_p1	ccsA-ndhD	1	TAAATTCCGCAATTGTGGCC	TCCGGATTTCGTTTTCTCACT	20	21	45.0	42.9	57.3	57.6	29.6	0.0	2.060087	121585	121860	474-474	1.000000	63.7
ccsA-ndhD_p2	ccsA-ndhD	2	AAATTCCGCAATTGTGGCCT	TCCGGATTTCGTTTTCTCACT	20	21	45.0	42.9	59.0	57.6	29.6	0.0	2.420373	121585	121860	473-473	1.000000	60.8
ccsA-ndhD_p3	ccsA-ndhD	3	TAAATTCCGCAATTGTGGCC	TCAGTTGAGAAGGTTGAAGCT	20	21	45.0	42.9	57.3	57.2	29.6	0.0	2.509607	121585	121860	452-452	1.000000	60.1
ccsA-ndhD_p4	ccsA-ndhD	4	GGGTTCCACAGTTATGGTTCA	TCCGGATTTCGTTTTCTCACT	21	21	47.6	42.9	57.9	57.6	0.0	0.0	2.545265	121585	121860	397-397	1.000000	59.8
ccsA-ndhD_p5	ccsA-ndhD	5	TAAATTCCGCAATTGTGGCC	TCCGGATTTCGTTTTCTCAC	20	20	45.0	45.0	57.3	56.1	29.6	0.0	2.575521	121585	121860	474-474	1.000000	59.6
psaC_p1	psaC	1	TAGAGTATTTTGAGCGCGGG	CTGGGAAATCATTGATTCATCTGG	20	24	50.0	41.7	57.8	57.9	8.8	12.2	4.304508	123499	123744	423-423	1.000000	45.8
psaC_p2	psaC	2	TAGAGTATTTTGAGCGCGGG	TGGGAAATCATTGATTCATCTGG	20	23	50.0	39.1	57.8	56.9	8.8	12.2	4.338450	123499	123744	422-422	1.000000	45.5
psaC_p3	psaC	3	TAGAGTATTTTGAGCGCGGG	GGGAAATCATTGATTCATCTGGT	20	23	50.0	39.1	57.8	56.9	8.8	12.2	4.340338	123499	123744	421-421	1.000000	45.5
psaC_p4	psaC	4	TAGAGTATTTTGAGCGCGGG	GGGAAATCATTGATTCATCTGGTG	20	24	50.0	41.7	57.8	58.2	8.8	12.2	4.379332	123499	123744	421-421	1.000000	45.2
psaC_p5	psaC	5	CTTGGACCAGAAAAACCCGT	CTGGGAAATCATTGATTCATCTGG	20	24	50.0	41.7	58.3	57.9	0.0	12.2	4.397992	123499	123744	452-452	1.000000	45.0