Pi
0.00298
MarkerSeek score
59.50
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | AAGAGATGCCGTTTCGAGTC | CCGAACCCATTCTGTCGATT | 50.0 | 50.0 | 58.0 | 58.0 | 1227-1743 bp | 1.0 | 82.0 |
| ycf1_p2 | CCGGAACTTCTTCGTAGTGG | CCGAACCCATTCTGTCGATT | 55.0 | 50.0 | 58.0 | 58.0 | 1356-1872 bp | 1.0 | 81.9 |
| ycf1_p3 | CCCGGAACTTCTTCGTAGTG | CCGAACCCATTCTGTCGATT | 55.0 | 50.0 | 58.0 | 58.0 | 1357-1873 bp | 1.0 | 81.8 |
| ycf1_p4 | GGCAGAATAGCAGAGCAAGT | CCGAACCCATTCTGTCGATT | 50.0 | 50.0 | 58.0 | 58.0 | 1738-2254 bp | 1.0 | 81.4 |
| ycf1_p5 | CCCGTAATTCTTCCTCAGCC | CCGAACCCATTCTGTCGATT | 55.0 | 50.0 | 58.0 | 58.0 | 1765-2281 bp | 1.0 | 81.3 |
| ycf1_p1 | ACGGACGATTCATGAATCATCA | GAATGGAAGCGGAATTCCCT | 40.9 | 50.0 | 57.9 | 57.9 | 343 bp | 1.0 | 66.1 |
| ycf1_p2 | GTTTCAACGGACGATTCATGA | GAATGGAAGCGGAATTCCCT | 42.9 | 50.0 | 56.9 | 57.9 | 349 bp | 1.0 | 65.9 |
| ycf1_p3 | AGTTTCAACGGACGATTCATG | GAATGGAAGCGGAATTCCCT | 42.9 | 50.0 | 56.9 | 57.9 | 350 bp | 1.0 | 65.8 |
| ycf1_p4 | TCAACGGACGATTCATGAATCA | GAATGGAAGCGGAATTCCCT | 40.9 | 50.0 | 58.1 | 57.9 | 346 bp | 1.0 | 65.4 |
| ycf1_p5 | TGGTATAGTTTCAACGGACGA | GAATGGAAGCGGAATTCCCT | 42.9 | 50.0 | 56.8 | 57.9 | 356 bp | 0.933333 | 61.2 |