Hotspot

rbcL

LSC · 56420-57847 · 1428 bp

Back to Fragaria

Pi 0.00593

MarkerSeek score 43.50

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 23
pi: 0.00593
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCAGTTGTAGGGAGGGACAT	ACTAAGAGTAAGTAATTGCTGGA	50.0	34.8	57.4	54.8	1471 bp	1.0	45.3
rbcL_p2	ATCAGTTGTAGGGAGGGACA	AGAACTAAGAGTAAGTAATTGCTGGA	50.0	34.6	57.4	57.9	1475 bp	1.0	45.2
rbcL_p3	TCAGTTGTAGGGAGGGACAT	AGAACTAAGAGTAAGTAATTGCTGGA	50.0	34.6	57.4	57.9	1474 bp	1.0	45.2
rbcL_p4	ATCAGTTGTAGGGAGGGACA	ACTAAGAGTAAGTAATTGCTGGA	50.0	34.8	57.4	54.8	1472 bp	1.0	45.2
rbcL_p5	TCAGTTGTAGGGAGGGACAT	GAACTAAGAGTAAGTAATTGCTGGA	50.0	36.0	57.4	56.6	1473 bp	1.0	45.2