Hotspot

ndhF-rpl32

SSC · 113789-114401 · 613 bp

Back to Fragaria

Pi 0.00674

MarkerSeek score 67.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 613
variable_sites: 22
pi: 0.00674
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAATGAACGGT	AAAAGCTTTCAATGCTGCCC	50.0	45.0	57.8	57.8	756-3000 bp	1.0	79.3
ndhF-rpl32_p2	CTGGAAGTGGAATGAACGGT	ATGCTGCCCAATACCCTTTC	50.0	50.0	57.8	58.2	745-3000 bp	1.0	79.0
ndhF-rpl32_p3	CTGGAAGTGGAATGAACGGT	AATGCTGCCCAATACCCTTT	50.0	45.0	57.8	57.7	746-3000 bp	1.0	78.7
ndhF-rpl32_p4	AAAGTAGAAGCCCTGCTCCT	AAAAGCTTTCAATGCTGCCC	50.0	45.0	58.3	57.8	789-3000 bp	1.0	78.0
ndhF-rpl32_p5	AAAGTAGAAGCCCTGCTCCT	ATGCTGCCCAATACCCTTTC	50.0	50.0	58.3	58.2	778-3000 bp	1.0	77.7