Hotspot

ycf1-ndhF

SSC · 111525-111544 · 20 bp

Back to Fragaria

Pi 0.0522

MarkerSeek score 61.10

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 20
variable_sites: 4
pi: 0.0522
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGAAGGGGACTAAACAGGA	CCGAACCCATTCTGTCGATT	50.0	50.0	58.0	58.0	290-806 bp	1.0	84.5
ycf1-ndhF_p2	CCTTCCCCTTCCCCTTTTTC	CCGAACCCATTCTGTCGATT	55.0	50.0	58.1	58.0	245-761 bp	1.0	84.5
ycf1-ndhF_p3	ACAGGAACAAGAGGGATCCA	CCGAACCCATTCTGTCGATT	50.0	50.0	58.0	58.0	276-792 bp	1.0	84.4
ycf1-ndhF_p4	GCAGAATACCGTCACCCATT	CCGAACCCATTCTGTCGATT	50.0	50.0	58.0	58.0	400-916 bp	1.0	83.7
ycf1-ndhF_p5	TGGGTTGGTATTAGGCTGGA	CCGAACCCATTCTGTCGATT	50.0	50.0	58.0	58.0	549-1065 bp	1.0	83.3