Hotspot

accD-psaI

LSC · 59996-60494 · 499 bp

Back to Fragaria

Pi 0.00542

MarkerSeek score 58.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 499
variable_sites: 12
pi: 0.00542
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAATCGTACCACGTAATCC	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	653-695 bp	1.0	73.8
accD-psaI_p2	CCAATCGTACCACGTAATCCT	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	652-694 bp	1.0	73.8
accD-psaI_p3	TCCAATCGTACCACGTAATCC	AGAAACCATTGCAATTGCCG	47.6	45.0	57.6	57.9	658-700 bp	1.0	73.3
accD-psaI_p4	TCCAATCGTACCACGTAATCC	TTGCCGGAAATACTAAGCCC	47.6	50.0	57.6	58.0	644-686 bp	0.933333	70.9
accD-psaI_p5	CCAATCGTACCACGTAATCCT	TTGCCGGAAATACTAAGCCC	47.6	50.0	57.6	58.0	643-685 bp	0.933333	70.8