Hotspot

rps4-trnT-UGU

LSC · 47311-47691 · 381 bp

Back to Fragaria

Pi 0.00698
MarkerSeek score 58.70
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
381
variable_sites
13
pi
0.00698
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 GATTGGGTTCGAAGATCGCT CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 550-553 bp 1.0 84.4
rps4-trnT-UGU_p2 CTTCCGGCTCTAGGCTTTTT CTCAGAGGTTAGAGCATCGC 50.0 55.0 57.9 57.9 532-535 bp 1.0 84.0
rps4-trnT-UGU_p3 GATTGGGTTCGAAGATCGCT ATGGTCATCGGTTCGATTCC 50.0 50.0 58.1 57.8 518-521 bp 1.0 83.6
rps4-trnT-UGU_p4 CTTCCGGCTCTAGGCTTTTT ATGGTCATCGGTTCGATTCC 50.0 50.0 57.9 57.8 500-503 bp 1.0 83.2
rps4-trnT-UGU_p5 CTAGTTAGCCCCGGTAAAGC CTCAGAGGTTAGAGCATCGC 55.0 55.0 57.8 57.9 511-514 bp 1.0 83.1