Hotspot

trnR-UCU-atpA

LSC · 10108-10720 · 613 bp

Back to Fragaria

Pi 0.0119

MarkerSeek score 53.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 613
variable_sites: 28
pi: 0.0119
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATTGGAGTGAAAAGCGTCCA	CGTTTTCTACTTCAGGAACAGG	45.0	45.5	57.7	57.0	713-860 bp	1.0	60.9
trnR-UCU-atpA_p2	TGGAGTGAAAAGCGTCCATT	CGTTTTCTACTTCAGGAACAGG	45.0	45.5	57.7	57.0	711-858 bp	1.0	60.9
trnR-UCU-atpA_p3	TTGGAGTGAAAAGCGTCCAT	CGTTTTCTACTTCAGGAACAGG	45.0	45.5	57.7	57.0	712-859 bp	1.0	60.9
trnR-UCU-atpA_p4	TGGAGTGAAAAGCGTCCATT	GGAACGTTTTCTACTTCAGGAAC	45.0	43.5	57.7	57.7	715-862 bp	1.0	58.1
trnR-UCU-atpA_p5	TTGGAGTGAAAAGCGTCCAT	GGAACGTTTTCTACTTCAGGAAC	45.0	43.5	57.7	57.7	716-863 bp	1.0	58.1