Hotspot

trnQ-UUG-psbK

LSC · 7220-7660 · 441 bp

Back to Fragaria

Pi 0.00523

MarkerSeek score 51.90

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 11
pi: 0.00523
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GACCAAAACCCGTTGCCTTA	CGTAAGCTTCGGGCAATTTG	50.0	50.0	58.7	58.1	570-724 bp	1.0	79.2
trnQ-UUG-psbK_p2	GACCAAAACCCGTTGCCTTA	GTAAGCTTCGGGCAATTTGG	50.0	50.0	58.7	57.7	569-723 bp	1.0	77.8
trnQ-UUG-psbK_p3	GAATAGCGGGACCAAAACCC	CGTAAGCTTCGGGCAATTTG	55.0	50.0	58.9	58.1	579-724 bp	1.0	77.4
trnQ-UUG-psbK_p4	AAAACCCGTTGCCTTACCAC	CGTAAGCTTCGGGCAATTTG	50.0	50.0	59.0	58.1	566-725 bp	1.0	76.9
trnQ-UUG-psbK_p5	ACCAAAACCCGTTGCCTTAC	CGTAAGCTTCGGGCAATTTG	50.0	50.0	59.0	58.1	569-725 bp	1.0	76.9