Hotspot

rpl23-trnM-CAU

Genome · 84583-84749 · 167 bp

Back to Fossombronia

Pi 0.0783
MarkerSeek score 76.70
Primer available yes
Label rpl23-trnM-CAU

Feature Metadata

label_name
rpl23-trnM-CAU
feature_type
igs
region
Genome
length_bp
167
variable_sites
13
pi
0.0783
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnM-CAU_p1 CTTTTCCAGCAAACGAATCGT GCTGAATGGTTAAAGCACCC 42.9 50.0 57.7 57.3 289-302 bp 1.0 68.4
rpl23-trnM-CAU_p2 CTTTTCCAGCAAACGAATCGT GGCTGAATGGTTAAAGCACC 42.9 50.0 57.7 57.3 290-303 bp 1.0 68.4
rpl23-trnM-CAU_p3 CTTTTCCAGCAAACGAATCGT TGGTTAAAGCACCCAACTCA 42.9 45.0 57.7 57.3 283-296 bp 1.0 67.8
rpl23-trnM-CAU_p4 CTTTTCCAGCAAACGAATCGT TGTTATGCATCCATGGCTGA 42.9 45.0 57.7 57.3 304-317 bp 1.0 67.6
rpl23-trnM-CAU_p5 TTTTCCAGCAAACGAATCGT GCTGAATGGTTAAAGCACCC 40.0 50.0 56.6 57.3 288-301 bp 1.0 67.1