Hotspot

trnW-trnP

Genome · 67519-67635 · 117 bp

Back to Fossombronia

Pi 0.12

MarkerSeek score 79.00

Primer available yes

Label trnW-trnP

Feature Metadata

label_name: trnW-trnP
feature_type: igs
region: Genome
length_bp: 117
variable_sites: 14
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnW-trnP_p1	GTTTTGGAGACCTGCGTTCT	GGTTCGAATCCTGTCATCCC	50.0	55.0	58.4	58.0	180-188 bp	1.0	82.5
trnW-trnP_p2	TTTGGAGACCTGCGTTCTAC	GGTTCGAATCCTGTCATCCC	50.0	55.0	57.5	58.0	178-186 bp	1.0	82.2
trnW-trnP_p3	GTTTTGGAGACCTGCGTTCT	GGATGTAGCGCAGTTTGGTA	50.0	50.0	58.4	58.0	230-238 bp	1.0	81.4
trnW-trnP_p4	TTTGGAGACCTGCGTTCTAC	GGATGTAGCGCAGTTTGGTA	50.0	50.0	57.5	58.0	228-236 bp	1.0	81.1
trnW-trnP_p5	GTTTTGGAGACCTGCGTTCT	GTTTGGTAGCGCGTTTGTTT	50.0	45.0	58.4	58.2	218-226 bp	1.0	80.4