Hotspot

psbE-petL

Genome · 66232-67006 · 775 bp

Back to Fossombronia

Pi 0.0932

MarkerSeek score 84.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: Genome
length_bp: 775
variable_sites: 70
pi: 0.0932
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	GGACGCTCTCCTGTATTTCC	GCTGAGGTTAGGACTCCGAT	55.0	55.0	57.8	58.6	847-854 bp	1.0	82.3
psbE-petL_p2	GGACGCTCTCCTGTATTTCC	ATGCTGAGGTTAGGACTCCG	55.0	55.0	57.8	58.9	849-856 bp	1.0	79.9
psbE-petL_p3	GGACGCTCTCCTGTATTTCC	GATGCTGAGGTTAGGACTCC	55.0	55.0	57.8	57.1	850-857 bp	1.0	79.8
psbE-petL_p4	GGACGCTCTCCTGTATTTCC	GCTGAGGTTAGGACTCCGA	55.0	57.9	57.8	58.1	847-854 bp	1.0	78.1
psbE-petL_p5	GGACGCTCTCCTGTATTTCC	ACTGATGCTGAGGTTAGGACT	55.0	47.6	57.8	58.2	853-860 bp	1.0	77.6