Hotspot

psbL-psbF

Genome · 65825-65848 · 24 bp

Back to Fossombronia

Pi 0.0476
MarkerSeek score 77.10
Primer available yes
Label psbL-psbF

Feature Metadata

label_name
psbL-psbF
feature_type
igs
region
Genome
length_bp
24
variable_sites
1
pi
0.0476
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbL-psbF_p1 CAACCCCCAATAGAGGCTTG GAGCTATATCGGCGATGCAA 55.0 50.0 58.2 58.0 122-125 bp 1.0 80.7
psbL-psbF_p2 CCCCAATAGAGGCTTGTACG GAGCTATATCGGCGATGCAA 55.0 50.0 57.8 58.0 118-121 bp 1.0 80.7
psbL-psbF_p3 ACCCCCAATAGAGGCTTGTA GAGCTATATCGGCGATGCAA 50.0 50.0 57.7 58.0 120-123 bp 1.0 80.4
psbL-psbF_p4 CAACCCCCAATAGAGGCTTG CTGTTCATGGATTGGCCGTA 55.0 50.0 58.2 58.0 161-164 bp 1.0 79.8
psbL-psbF_p5 CCCCAATAGAGGCTTGTACG CTGTTCATGGATTGGCCGTA 55.0 50.0 57.8 58.0 157-160 bp 1.0 79.8