Hotspot

atpB-rbcL

Genome · 58009-58622 · 614 bp

Back to Fossombronia

Pi 0.112

MarkerSeek score 84.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 614
variable_sites: 59
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	AAGCACGGAAGCTCCAAAAA	AGTTTCCGTTTGTGGTGACA	45.0	45.0	58.6	57.9	663-674 bp	1.0	85.3
atpB-rbcL_p2	AGCACGGAAGCTCCAAAAA	AGTTTCCGTTTGTGGTGACA	47.4	45.0	57.9	57.9	662-673 bp	1.0	81.3
atpB-rbcL_p3	AAGCACGGAAGCTCCAAAA	AGTTTCCGTTTGTGGTGACA	47.4	45.0	57.9	57.9	663-674 bp	1.0	81.2
atpB-rbcL_p4	AAGCACGGAAGCTCCAAAAA	ACACCTGCTTTAGTTTCCGT	45.0	45.0	58.6	57.4	674-685 bp	1.0	81.1
atpB-rbcL_p5	CAAGCACGGAAGCTCCAAAA	AGTTTCCGTTTGTGGTGACA	50.0	45.0	59.3	57.9	664-675 bp	1.0	79.5