Hotspot

trnL-UAA-trnF-GAA

Genome · 52807-52959 · 153 bp

Back to Fossombronia

Pi 0.116

MarkerSeek score 81.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: Genome
length_bp: 153
variable_sites: 17
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	GAAAATCCGTTGGCTTGACC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.7	58.1	259-263 bp	1.0	83.9
trnL-UAA-trnF-GAA_p2	GGAGAAAATCCGTTGGCTTG	TTTGAACTGGTGACACGAGG	50.0	50.0	57.4	58.1	262-266 bp	1.0	81.5
trnL-UAA-trnF-GAA_p3	GAAAATCCGTTGGCTTGACC	GAACTGGTGACACGAGGATT	50.0	50.0	57.7	57.5	256-260 bp	1.0	80.7
trnL-UAA-trnF-GAA_p4	GAAAATCCGTTGGCTTGACC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.7	58.6	221-225 bp	1.0	80.7
trnL-UAA-trnF-GAA_p5	CGATGCAAATCGTGGTAAGG	TTTGAACTGGTGACACGAGG	50.0	50.0	57.3	58.1	280-284 bp	1.0	80.2