Hotspot

trnS-GGA-rps4

Genome · 50915-51157 · 243 bp

Back to Fossombronia

Pi 0.0928

MarkerSeek score 77.70

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: Genome
length_bp: 243
variable_sites: 22
pi: 0.0928
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	TTCTATGCCTACCGAGGGTT	GTCGTGGAGTATTATTCTCGTCA	50.0	43.5	57.8	57.8	309-311 bp	1.0	60.5
trnS-GGA-rps4_p2	TTTCTATGCCTACCGAGGGT	GTCGTGGAGTATTATTCTCGTCA	50.0	43.5	57.8	57.8	310-312 bp	1.0	60.5
trnS-GGA-rps4_p3	AGGTGGTTGAGCGGTTTAAG	GTCGTGGAGTATTATTCTCGTCA	50.0	43.5	58.1	57.8	358-360 bp	1.0	60.0
trnS-GGA-rps4_p4	GGTGGTTGAGCGGTTTAAGA	GTCGTGGAGTATTATTCTCGTCA	50.0	43.5	58.1	57.8	357-359 bp	1.0	60.0
trnS-GGA-rps4_p5	TCTATGCCTACCGAGGGTTC	GTCGTGGAGTATTATTCTCGTCA	55.0	43.5	58.3	57.8	308-310 bp	1.0	59.5