Hotspot

trnF-GAA-psbD

Genome · 39031-39754 · 724 bp

Back to Fossombronia

Pi 0.0617

MarkerSeek score 81.10

Primer available yes

Label trnF-GAA-psbD

Feature Metadata

label_name: trnF-GAA-psbD
feature_type: igs
region: Genome
length_bp: 724
variable_sites: 44
pi: 0.0617
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-psbD_p1	GTGGTAGAGTAACGCCATGG	GAATACGAAACGGTCCCTCC	55.0	55.0	58.1	58.1	872-876 bp	1.0	84.0
trnF-GAA-psbD_p2	GTGGTAGAGTAACGCCATGG	GCCTACGAATACGAAACGGT	55.0	50.0	58.1	58.1	878-882 bp	1.0	83.8
trnF-GAA-psbD_p3	TCAGTGGTAGAGTAACGCCA	GAATACGAAACGGTCCCTCC	50.0	55.0	58.1	58.1	875-879 bp	1.0	83.8
trnF-GAA-psbD_p4	GTCACCGGTTCAAATCTGGT	GAATACGAAACGGTCCCTCC	50.0	55.0	58.1	58.1	842-846 bp	1.0	83.8
trnF-GAA-psbD_p5	TCAGTGGTAGAGTAACGCCA	GCCTACGAATACGAAACGGT	50.0	50.0	58.1	58.1	881-885 bp	1.0	83.6