Hotspot

trnE-UUC-trnF-GAA

Genome · 38418-38957 · 540 bp

Back to Fossombronia

Pi 0.0633
MarkerSeek score 80.50
Primer available yes
Label trnE-UUC-trnF-GAA

Feature Metadata

label_name
trnE-UUC-trnF-GAA
feature_type
igs
region
Genome
length_bp
540
variable_sites
34
pi
0.0633
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnF-GAA_p1 CCGTCACCTCCTTGAAAGAG CCATGGCGTTACTCTACCAC 55.0 55.0 57.9 58.1 625-629 bp 1.0 83.5
trnE-UUC-trnF-GAA_p2 CCGTCACCTCCTTGAAAGAG TGGCGTTACTCTACCACTGA 55.0 50.0 57.9 58.1 622-626 bp 1.0 83.4
trnE-UUC-trnF-GAA_p3 CCGTCACCTCCTTGAAAGAG ACCAGATTTGAACCGGTGAC 55.0 50.0 57.9 58.1 655-659 bp 1.0 83.0
trnE-UUC-trnF-GAA_p4 CCGTCACCTCCTTGAAAGAG GACAAAGAGCCCCTTACCAG 55.0 55.0 57.9 57.9 670-674 bp 1.0 82.8
trnE-UUC-trnF-GAA_p5 CCGTCACCTCCTTGAAAGAG ACATGGATGACAAAGAGCCC 55.0 50.0 57.9 57.9 678 bp 0.5 58.2