Hotspot

trnG-GCC-trnY-GUA

Genome · 38038-38191 · 154 bp

Back to Fossombronia

Pi 0.11

MarkerSeek score 82.20

Primer available yes

Label trnG-GCC-trnY-GUA

Feature Metadata

label_name: trnG-GCC-trnY-GUA
feature_type: igs
region: Genome
length_bp: 154
variable_sites: 16
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnY-GUA_p1	TTGACAGGGCGGTACTCTAA	CCTACGCTGGTTCAAATCCA	50.0	50.0	58.1	57.9	217-225 bp	1.0	87.2
trnG-GCC-trnY-GUA_p2	CTTGACAGGGCGGTACTCTA	CCTACGCTGGTTCAAATCCA	55.0	50.0	58.5	57.9	218-226 bp	1.0	83.6
trnG-GCC-trnY-GUA_p3	CAACTTCCGTCTTGACAGGG	CCTACGCTGGTTCAAATCCA	55.0	50.0	58.5	57.9	228-236 bp	1.0	83.5
trnG-GCC-trnY-GUA_p4	TTGACAGGGCGGTACTCTAA	GACTGTAAATCCGCTGGCAA	50.0	50.0	58.1	58.6	239-247 bp	1.0	82.9
trnG-GCC-trnY-GUA_p5	TTGACAGGGCGGTACTCTAA	ACTGTAAATCCGCTGGCAAC	50.0	50.0	58.1	58.8	238-246 bp	1.0	80.7