Hotspot

chlB-trnK-UUU

Genome · 26719-27346 · 628 bp

Back to Fossombronia

Pi 0.0747

MarkerSeek score 77.90

Primer available yes

Label chlB-trnK-UUU

Feature Metadata

label_name: chlB-trnK-UUU
feature_type: igs
region: Genome
length_bp: 628
variable_sites: 46
pi: 0.0747
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-trnK-UUU_p1	AATCGCGGACCTGGTCTATA	AGAGACGAACGAACGACATG	50.0	50.0	58.0	58.0	1065-1073 bp	1.0	85.0
chlB-trnK-UUU_p2	ATCGCGGACCTGGTCTATAA	AGAGACGAACGAACGACATG	50.0	50.0	58.0	58.0	1064-1072 bp	1.0	85.0
chlB-trnK-UUU_p3	TCGCGGACCTGGTCTATAAT	AGAGACGAACGAACGACATG	50.0	50.0	58.0	58.0	1063-1071 bp	1.0	85.0
chlB-trnK-UUU_p4	AAGCTGTGGTTTTCGGTGAT	AGAGACGAACGAACGACATG	45.0	50.0	58.0	58.0	1411-1419 bp	1.0	83.9
chlB-trnK-UUU_p5	GTTGGAATCCTGAGAGCCAG	AGAGACGAACGAACGACATG	55.0	50.0	58.0	58.0	951 bp	0.5	59.4