Hotspot

ndhB-trnL-CAA

Genome · 3807-4034 · 228 bp

Back to Fossombronia

Pi 0.196

MarkerSeek score 77.20

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: Genome
length_bp: 228
variable_sites: 43
pi: 0.196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	CACCTTGGGTTTTCTCACGA	GATAACCTCCCTCCCCTACC	50.0	60.0	58.0	58.0	869 bp	0.5	59.5
ndhB-trnL-CAA_p2	TCACCTTGGGTTTTCTCACG	GATAACCTCCCTCCCCTACC	50.0	60.0	58.0	58.0	870 bp	0.5	59.5
ndhB-trnL-CAA_p3	CACCTTGGGTTTTCTCACGA	TGTTTTGACTAGCTGTGCGT	50.0	45.0	58.0	58.1	831 bp	0.5	59.3
ndhB-trnL-CAA_p4	TCACCTTGGGTTTTCTCACG	TGTTTTGACTAGCTGTGCGT	50.0	45.0	58.0	58.1	832 bp	0.5	59.3
ndhB-trnL-CAA_p5	CACCTTGGGTTTTCTCACGA	TAGTAATACGTCAGCCCGGT	50.0	50.0	58.0	57.9	651 bp	0.5	59.2