Hotspot

23rrn

Genome · 115848-118655 · 2808 bp

Back to Fossombronia

Pi 0.0453

MarkerSeek score 68.10

Primer available yes

Label 23rrn

Feature Metadata

label_name: 23rrn
feature_type: rRNA
region: Genome
length_bp: 2808
variable_sites: 127
pi: 0.0453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
23rrn_p1	GTCTGTTAGGATGCCTCAGC	AGCATAGCTTCGAATGGCAA	55.0	45.0	58.1	58.0	2992-2998 bp	1.0	82.9
23rrn_p2	TTGCCGTGACCTTATCTTGG	AGCATAGCTTCGAATGGCAA	50.0	45.0	57.9	58.0	2917-2923 bp	1.0	82.5
23rrn_p3	TCTGTTAGGATGCCTCAGCT	AGCATAGCTTCGAATGGCAA	50.0	45.0	58.1	58.0	2991-2997 bp	1.0	82.3
23rrn_p4	TGGATTCCAAAGCCCATCAG	AGCATAGCTTCGAATGGCAA	50.0	45.0	57.9	58.0	2900-2906 bp	1.0	82.2
23rrn_p5	GGATTCCAAAGCCCATCAGT	AGCATAGCTTCGAATGGCAA	50.0	45.0	57.9	58.0	2899-2905 bp	1.0	82.2