Hotspot

16rrn

Genome · 85494-86983 · 1490 bp

Back to Fossombronia

Pi 0.0612

MarkerSeek score 75.40

Primer available yes

Label 16rrn

Feature Metadata

label_name: 16rrn
feature_type: rRNA
region: Genome
length_bp: 1490
variable_sites: 91
pi: 0.0612
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
16rrn_p1	CCCGCTATCAAAAGAGGCTT	TCGTTAGCACCCAGGTAGAA	50.0	50.0	58.0	58.1	1852-1855 bp	1.0	83.5
16rrn_p2	CCCGCTATCAAAAGAGGCTT	AACAAAAGGAATGCGCCATG	50.0	45.0	58.0	57.9	2090-2093 bp	1.0	83.1
16rrn_p3	CCCGCTATCAAAAGAGGCTT	ATGCGTTATCAGGTGCTCTG	50.0	50.0	58.0	58.1	2329-2332 bp	1.0	82.9
16rrn_p4	CCCGCTATCAAAAGAGGCTT	TCTTGGTCTGTGAAGATGCG	50.0	50.0	58.0	57.9	2344-2347 bp	1.0	82.7
16rrn_p5	CCCGCTATCAAAAGAGGCTT	GTGATCTCGCAGTTCTTGGT	50.0	50.0	58.0	57.9	2357-2360 bp	1.0	82.6