primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	CACCTTGGGTTTTCTCACGA	GATAACCTCCCTCCCCTACC	20	20	50.0	60.0	58.0	58.0	0.0	0.0	0.065304	3807	4034	869-869	0.500000	59.5
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	TCACCTTGGGTTTTCTCACG	GATAACCTCCCTCCCCTACC	20	20	50.0	60.0	58.0	58.0	0.0	0.0	0.065304	3807	4034	870-870	0.500000	59.5
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	CACCTTGGGTTTTCTCACGA	TGTTTTGACTAGCTGTGCGT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.090102	3807	4034	831-831	0.500000	59.3
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	TCACCTTGGGTTTTCTCACG	TGTTTTGACTAGCTGTGCGT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.090102	3807	4034	832-832	0.500000	59.3
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	CACCTTGGGTTTTCTCACGA	TAGTAATACGTCAGCCCGGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.095344	3807	4034	651-651	0.500000	59.2
chlB-trnK-UUU_p1	chlB-trnK-UUU	1	AATCGCGGACCTGGTCTATA	AGAGACGAACGAACGACATG	20	20	50.0	50.0	58.0	58.0	25.0	12.0	0.043796	26719	27346	1065-1073	1.000000	85.0
chlB-trnK-UUU_p2	chlB-trnK-UUU	2	ATCGCGGACCTGGTCTATAA	AGAGACGAACGAACGACATG	20	20	50.0	50.0	58.0	58.0	25.0	12.0	0.043796	26719	27346	1064-1072	1.000000	85.0
chlB-trnK-UUU_p3	chlB-trnK-UUU	3	TCGCGGACCTGGTCTATAAT	AGAGACGAACGAACGACATG	20	20	50.0	50.0	58.0	58.0	25.0	12.0	0.043796	26719	27346	1063-1071	1.000000	85.0
chlB-trnK-UUU_p4	chlB-trnK-UUU	4	AAGCTGTGGTTTTCGGTGAT	AGAGACGAACGAACGACATG	20	20	45.0	50.0	58.0	58.0	0.0	12.0	0.053660	26719	27346	1411-1419	1.000000	83.9
chlB-trnK-UUU_p5	chlB-trnK-UUU	5	GTTGGAATCCTGAGAGCCAG	AGAGACGAACGAACGACATG	20	20	55.0	50.0	58.0	58.0	0.0	12.0	0.070447	26719	27346	951-951	0.500000	59.4
ycf2-trnG-GCC_p1	ycf2-trnG-GCC	1	GTTTGTTCCCACAGATCCGT	CTTTCTATCTGGGTCGGTGC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.166466	37621	37959	1225-1232	1.000000	85.7
ycf2-trnG-GCC_p2	ycf2-trnG-GCC	2	GTTTGTTCCCACAGATCCGT	CCCCCACGATCGATGATTTT	20	20	50.0	50.0	58.1	58.0	0.0	24.3	0.140472	37621	37959	1647-1650	1.000000	85.1
ycf2-trnG-GCC_p3	ycf2-trnG-GCC	3	TAAGGGGGAGGAACGTACAG	CCCCCACGATCGATGATTTT	20	20	55.0	50.0	58.1	58.0	0.0	24.3	0.176053	37621	37959	1818-1821	1.000000	84.4
ycf2-trnG-GCC_p4	ycf2-trnG-GCC	4	GTTTGTTCCCACAGATCCGT	GAGCTCCAAATGGTCCAGAG	20	20	50.0	55.0	58.1	58.0	0.0	0.9	0.143321	37621	37959	1109-1109	0.500000	58.9
ycf2-trnG-GCC_p5	ycf2-trnG-GCC	5	TAAGGGGGAGGAACGTACAG	GAGCTCCAAATGGTCCAGAG	20	20	55.0	55.0	58.1	58.0	0.0	0.9	0.178901	37621	37959	1280-1280	0.500000	58.6
trnG-GCC-trnY-GUA_p1	trnG-GCC-trnY-GUA	1	TTGACAGGGCGGTACTCTAA	CCTACGCTGGTTCAAATCCA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180463	38038	38191	217-225	1.000000	87.2
trnG-GCC-trnY-GUA_p2	trnG-GCC-trnY-GUA	2	CTTGACAGGGCGGTACTCTA	CCTACGCTGGTTCAAATCCA	20	20	55.0	50.0	58.5	57.9	0.0	0.0	0.636831	38038	38191	218-226	1.000000	83.6
trnG-GCC-trnY-GUA_p3	trnG-GCC-trnY-GUA	3	CAACTTCCGTCTTGACAGGG	CCTACGCTGGTTCAAATCCA	20	20	55.0	50.0	58.5	57.9	1.6	0.0	0.595024	38038	38191	228-236	1.000000	83.5
trnG-GCC-trnY-GUA_p4	trnG-GCC-trnY-GUA	4	TTGACAGGGCGGTACTCTAA	GACTGTAAATCCGCTGGCAA	20	20	50.0	50.0	58.1	58.6	0.0	0.0	0.623528	38038	38191	239-247	1.000000	82.9
trnG-GCC-trnY-GUA_p5	trnG-GCC-trnY-GUA	5	TTGACAGGGCGGTACTCTAA	ACTGTAAATCCGCTGGCAAC	20	20	50.0	50.0	58.1	58.8	0.0	0.0	0.907243	38038	38191	238-246	1.000000	80.7
trnE-UUC-trnF-GAA_p1	trnE-UUC-trnF-GAA	1	CCGTCACCTCCTTGAAAGAG	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	57.9	58.1	0.0	0.0	0.150122	38418	38957	625-629	1.000000	83.5
trnE-UUC-trnF-GAA_p2	trnE-UUC-trnF-GAA	2	CCGTCACCTCCTTGAAAGAG	TGGCGTTACTCTACCACTGA	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.172276	38418	38957	622-626	1.000000	83.4
trnE-UUC-trnF-GAA_p3	trnE-UUC-trnF-GAA	3	CCGTCACCTCCTTGAAAGAG	ACCAGATTTGAACCGGTGAC	20	20	55.0	50.0	57.9	58.1	0.0	8.5	0.193845	38418	38957	655-659	1.000000	83.0
trnE-UUC-trnF-GAA_p4	trnE-UUC-trnF-GAA	4	CCGTCACCTCCTTGAAAGAG	GACAAAGAGCCCCTTACCAG	20	20	55.0	55.0	57.9	57.9	0.0	0.0	0.201397	38418	38957	670-674	1.000000	82.8
trnE-UUC-trnF-GAA_p5	trnE-UUC-trnF-GAA	5	CCGTCACCTCCTTGAAAGAG	ACATGGATGACAAAGAGCCC	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.220843	38418	38957	678-678	0.500000	58.2
trnF-GAA-psbD_p1	trnF-GAA-psbD	1	GTGGTAGAGTAACGCCATGG	GAATACGAAACGGTCCCTCC	20	20	55.0	55.0	58.1	58.1	7.6	0.0	0.132161	39031	39754	872-876	1.000000	84.0
trnF-GAA-psbD_p2	trnF-GAA-psbD	2	GTGGTAGAGTAACGCCATGG	GCCTACGAATACGAAACGGT	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.150751	39031	39754	878-882	1.000000	83.8
trnF-GAA-psbD_p3	trnF-GAA-psbD	3	TCAGTGGTAGAGTAACGCCA	GAATACGAAACGGTCCCTCC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.154316	39031	39754	875-879	1.000000	83.8
trnF-GAA-psbD_p4	trnF-GAA-psbD	4	GTCACCGGTTCAAATCTGGT	GAATACGAAACGGTCCCTCC	20	20	50.0	55.0	58.1	58.1	3.0	0.0	0.175884	39031	39754	842-846	1.000000	83.8
trnF-GAA-psbD_p5	trnF-GAA-psbD	5	TCAGTGGTAGAGTAACGCCA	GCCTACGAATACGAAACGGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.172905	39031	39754	881-885	1.000000	83.6
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	TTCTATGCCTACCGAGGGTT	GTCGTGGAGTATTATTCTCGTCA	20	23	50.0	43.5	57.8	57.8	0.0	0.0	3.335923	50915	51157	309-311	1.000000	60.5
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	TTTCTATGCCTACCGAGGGT	GTCGTGGAGTATTATTCTCGTCA	20	23	50.0	43.5	57.8	57.8	0.0	0.0	3.335923	50915	51157	310-312	1.000000	60.5
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	AGGTGGTTGAGCGGTTTAAG	GTCGTGGAGTATTATTCTCGTCA	20	23	50.0	43.5	58.1	57.8	0.0	0.0	3.273702	50915	51157	358-360	1.000000	60.0
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	GGTGGTTGAGCGGTTTAAGA	GTCGTGGAGTATTATTCTCGTCA	20	23	50.0	43.5	58.1	57.8	0.0	0.0	3.279025	50915	51157	357-359	1.000000	60.0
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	TCTATGCCTACCGAGGGTTC	GTCGTGGAGTATTATTCTCGTCA	20	23	55.0	43.5	58.3	57.8	0.0	0.0	3.464956	50915	51157	308-310	1.000000	59.5
trnL-UAA-trnF-GAA_p1	trnL-UAA-trnF-GAA	1	GAAAATCCGTTGGCTTGACC	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.7	58.1	0.0	20.1	0.399593	52807	52959	259-263	1.000000	83.9
trnL-UAA-trnF-GAA_p2	trnL-UAA-trnF-GAA	2	GGAGAAAATCCGTTGGCTTG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.4	58.1	0.0	20.1	0.688664	52807	52959	262-266	1.000000	81.5
trnL-UAA-trnF-GAA_p3	trnL-UAA-trnF-GAA	3	GAAAATCCGTTGGCTTGACC	GAACTGGTGACACGAGGATT	20	20	50.0	50.0	57.7	57.5	0.0	20.1	0.805063	52807	52959	256-260	1.000000	80.7
trnL-UAA-trnF-GAA_p4	trnL-UAA-trnF-GAA	4	GAAAATCCGTTGGCTTGACC	TACCAACTGAGCTATCCCGG	20	20	50.0	55.0	57.7	58.6	0.0	0.0	0.937693	52807	52959	221-225	1.000000	80.7
trnL-UAA-trnF-GAA_p5	trnL-UAA-trnF-GAA	5	CGATGCAAATCGTGGTAAGG	TTTGAACTGGTGACACGAGG	20	20	50.0	50.0	57.3	58.1	1.5	20.1	0.789363	52807	52959	280-284	1.000000	80.2
atpB-rbcL_p1	atpB-rbcL	1	AAGCACGGAAGCTCCAAAAA	AGTTTCCGTTTGTGGTGACA	20	20	45.0	45.0	58.6	57.9	0.0	0.0	0.718135	58009	58622	663-674	1.000000	85.3
atpB-rbcL_p2	atpB-rbcL	2	AGCACGGAAGCTCCAAAAA	AGTTTCCGTTTGTGGTGACA	19	20	47.4	45.0	57.9	57.9	0.0	0.0	1.226674	58009	58622	662-673	1.000000	81.3
atpB-rbcL_p3	atpB-rbcL	3	AAGCACGGAAGCTCCAAAA	AGTTTCCGTTTGTGGTGACA	19	20	47.4	45.0	57.9	57.9	0.0	0.0	1.226674	58009	58622	663-674	1.000000	81.2
atpB-rbcL_p4	atpB-rbcL	4	AAGCACGGAAGCTCCAAAAA	ACACCTGCTTTAGTTTCCGT	20	20	45.0	45.0	58.6	57.4	0.0	0.0	1.234294	58009	58622	674-685	1.000000	81.1
atpB-rbcL_p5	atpB-rbcL	5	CAAGCACGGAAGCTCCAAAA	AGTTTCCGTTTGTGGTGACA	20	20	50.0	45.0	59.3	57.9	0.0	0.0	1.448834	58009	58622	664-675	1.000000	79.5
psbL-psbF_p1	psbL-psbF	1	CAACCCCCAATAGAGGCTTG	GAGCTATATCGGCGATGCAA	20	20	55.0	50.0	58.2	58.0	0.0	31.1	0.235950	65825	65848	122-125	1.000000	80.7
psbL-psbF_p2	psbL-psbF	2	CCCCAATAGAGGCTTGTACG	GAGCTATATCGGCGATGCAA	20	20	55.0	50.0	57.8	58.0	0.0	31.1	0.244202	65825	65848	118-121	1.000000	80.7
psbL-psbF_p3	psbL-psbF	3	ACCCCCAATAGAGGCTTGTA	GAGCTATATCGGCGATGCAA	20	20	50.0	50.0	57.7	58.0	0.0	31.1	0.284016	65825	65848	120-123	1.000000	80.4
psbL-psbF_p4	psbL-psbF	4	CAACCCCCAATAGAGGCTTG	CTGTTCATGGATTGGCCGTA	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.269199	65825	65848	161-164	1.000000	79.8
psbL-psbF_p5	psbL-psbF	5	CCCCAATAGAGGCTTGTACG	CTGTTCATGGATTGGCCGTA	20	20	55.0	50.0	57.8	58.0	0.0	0.0	0.277451	65825	65848	157-160	1.000000	79.8
psbE-petL_p1	psbE-petL	1	GGACGCTCTCCTGTATTTCC	GCTGAGGTTAGGACTCCGAT	20	20	55.0	55.0	57.8	58.6	0.0	0.0	0.821759	66232	67006	847-854	1.000000	82.3
psbE-petL_p2	psbE-petL	2	GGACGCTCTCCTGTATTTCC	ATGCTGAGGTTAGGACTCCG	20	20	55.0	55.0	57.8	58.9	0.0	0.0	1.111135	66232	67006	849-856	1.000000	79.9
psbE-petL_p3	psbE-petL	3	GGACGCTCTCCTGTATTTCC	GATGCTGAGGTTAGGACTCC	20	20	55.0	55.0	57.8	57.1	0.0	0.0	1.127318	66232	67006	850-857	1.000000	79.8
psbE-petL_p4	psbE-petL	4	GGACGCTCTCCTGTATTTCC	GCTGAGGTTAGGACTCCGA	20	19	55.0	57.9	57.8	58.1	0.0	0.0	1.343030	66232	67006	847-854	1.000000	78.1
psbE-petL_p5	psbE-petL	5	GGACGCTCTCCTGTATTTCC	ACTGATGCTGAGGTTAGGACT	20	21	55.0	47.6	57.8	58.2	0.0	0.0	1.400114	66232	67006	853-860	1.000000	77.6
trnW-trnP_p1	trnW-trnP	1	GTTTTGGAGACCTGCGTTCT	GGTTCGAATCCTGTCATCCC	20	20	50.0	55.0	58.4	58.0	0.0	0.0	0.450742	67519	67635	180-188	1.000000	82.5
trnW-trnP_p2	trnW-trnP	2	TTTGGAGACCTGCGTTCTAC	GGTTCGAATCCTGTCATCCC	20	20	50.0	55.0	57.5	58.0	0.0	0.0	0.495682	67519	67635	178-186	1.000000	82.2
trnW-trnP_p3	trnW-trnP	3	GTTTTGGAGACCTGCGTTCT	GGATGTAGCGCAGTTTGGTA	20	20	50.0	50.0	58.4	58.0	0.0	11.5	0.424211	67519	67635	230-238	1.000000	81.4
trnW-trnP_p4	trnW-trnP	4	TTTGGAGACCTGCGTTCTAC	GGATGTAGCGCAGTTTGGTA	20	20	50.0	50.0	57.5	58.0	0.0	11.5	0.469151	67519	67635	228-236	1.000000	81.1
trnW-trnP_p5	trnW-trnP	5	GTTTTGGAGACCTGCGTTCT	GTTTGGTAGCGCGTTTGTTT	20	20	50.0	45.0	58.4	58.2	0.0	8.4	0.575583	67519	67635	218-226	1.000000	80.4
rpl23-trnM-CAU_p1	rpl23-trnM-CAU	1	CTTTTCCAGCAAACGAATCGT	GCTGAATGGTTAAAGCACCC	21	20	42.9	50.0	57.7	57.3	1.0	0.0	1.922762	84583	84749	289-302	1.000000	68.4
rpl23-trnM-CAU_p2	rpl23-trnM-CAU	2	CTTTTCCAGCAAACGAATCGT	GGCTGAATGGTTAAAGCACC	21	20	42.9	50.0	57.7	57.3	1.0	0.0	1.922762	84583	84749	290-303	1.000000	68.4
rpl23-trnM-CAU_p3	rpl23-trnM-CAU	3	CTTTTCCAGCAAACGAATCGT	TGGTTAAAGCACCCAACTCA	21	20	42.9	45.0	57.7	57.3	1.0	0.0	2.006599	84583	84749	283-296	1.000000	67.8
rpl23-trnM-CAU_p4	rpl23-trnM-CAU	4	CTTTTCCAGCAAACGAATCGT	TGTTATGCATCCATGGCTGA	21	20	42.9	45.0	57.7	57.3	1.0	19.8	2.000856	84583	84749	304-317	1.000000	67.6
rpl23-trnM-CAU_p5	rpl23-trnM-CAU	5	TTTTCCAGCAAACGAATCGT	GCTGAATGGTTAAAGCACCC	20	20	40.0	50.0	56.6	57.3	1.0	0.0	2.087265	84583	84749	288-301	1.000000	67.1
trnM-CAU-trnT_p1	trnM-CAU-trnT	1	GGTGCTTTAACCATTCAGCC	GACGACTTCCACCATGTCAA	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.818567	84824	85198	453-521	1.000000	80.4
trnM-CAU-trnT_p2	trnM-CAU-trnT	2	GGGTGCTTTAACCATTCAGC	GACGACTTCCACCATGTCAA	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.818567	84824	85198	454-522	1.000000	80.3
trnM-CAU-trnT_p3	trnM-CAU-trnT	3	GGGTGCTTTAACCATTCAGC	ACGACTTCCACCATGTCAAG	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.824582	84824	85198	453-521	1.000000	80.3
trnM-CAU-trnT_p4	trnM-CAU-trnT	4	GGTGCTTTAACCATTCAGCC	ACGACTTCCACCATGTCAAG	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.824582	84824	85198	452-520	1.000000	80.3
trnM-CAU-trnT_p5	trnM-CAU-trnT	5	GGTGCTTTAACCATTCAGCC	AACTGACGACTTCCACCATG	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.824582	84824	85198	457-525	1.000000	80.2
16rrn_p1	16rrn	1	CCCGCTATCAAAAGAGGCTT	TCGTTAGCACCCAGGTAGAA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.116635	85494	86983	1852-1855	1.000000	83.5
16rrn_p2	16rrn	2	CCCGCTATCAAAAGAGGCTT	AACAAAAGGAATGCGCCATG	20	20	50.0	45.0	58.0	57.9	0.0	4.2	0.127289	85494	86983	2090-2093	1.000000	83.1
16rrn_p3	16rrn	3	CCCGCTATCAAAAGAGGCTT	ATGCGTTATCAGGTGCTCTG	20	20	50.0	50.0	58.0	58.1	0.0	1.9	0.098735	85494	86983	2329-2332	1.000000	82.9
16rrn_p4	16rrn	4	CCCGCTATCAAAAGAGGCTT	TCTTGGTCTGTGAAGATGCG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.128732	85494	86983	2344-2347	1.000000	82.7
16rrn_p5	16rrn	5	CCCGCTATCAAAAGAGGCTT	GTGATCTCGCAGTTCTTGGT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.130177	85494	86983	2357-2360	1.000000	82.6
rpl32-trnP_p1	rpl32-trnP	1	GGATGGTAAACCGCTCGATT	CATCGCGCTCGGAATAGTTA	20	20	50.0	50.0	58.1	58.0	0.0	5.6	0.096817	96988	98037	1727-1819	1.000000	90.5
rpl32-trnP_p2	rpl32-trnP	2	GGATGGTAAACCGCTCGATT	TAACATCGCGCTCGGAATAG	20	20	50.0	50.0	58.1	58.0	0.0	5.6	0.096817	96988	98037	1730-1822	1.000000	90.5
rpl32-trnP_p3	rpl32-trnP	3	ACTCTTTCCCGGTGTGTTTC	CATCGCGCTCGGAATAGTTA	20	20	50.0	50.0	58.0	58.0	0.0	5.6	0.083110	96988	98037	1923-2015	1.000000	90.4
rpl32-trnP_p4	rpl32-trnP	4	ACTCTTTCCCGGTGTGTTTC	TAACATCGCGCTCGGAATAG	20	20	50.0	50.0	58.0	58.0	0.0	5.6	0.083110	96988	98037	1926-2018	1.000000	90.4
rpl32-trnP_p5	rpl32-trnP	5	ACTCTTTCCCGGTGTGTTTC	CTAACATCGCGCTCGGAATA	20	20	50.0	50.0	58.0	58.0	0.0	5.6	0.083110	96988	98037	1927-2019	1.000000	90.3
23rrn_p1	23rrn	1	GTCTGTTAGGATGCCTCAGC	AGCATAGCTTCGAATGGCAA	20	20	55.0	45.0	58.1	58.0	0.0	11.5	0.095941	115848	118655	2992-2998	1.000000	82.9
23rrn_p2	23rrn	2	TTGCCGTGACCTTATCTTGG	AGCATAGCTTCGAATGGCAA	20	20	50.0	45.0	57.9	58.0	0.0	11.5	0.150636	115848	118655	2917-2923	1.000000	82.5
23rrn_p3	23rrn	3	TCTGTTAGGATGCCTCAGCT	AGCATAGCTTCGAATGGCAA	20	20	50.0	45.0	58.1	58.0	0.0	11.5	0.171268	115848	118655	2991-2997	1.000000	82.3
23rrn_p4	23rrn	4	TGGATTCCAAAGCCCATCAG	AGCATAGCTTCGAATGGCAA	20	20	50.0	45.0	57.9	58.0	1.9	11.5	0.191132	115848	118655	2900-2906	1.000000	82.2
23rrn_p5	23rrn	5	GGATTCCAAAGCCCATCAGT	AGCATAGCTTCGAATGGCAA	20	20	50.0	45.0	57.9	58.0	0.0	11.5	0.192659	115848	118655	2899-2905	1.000000	82.2