Hotspot

ycf1

SSC · 126180-131795 · 5616 bp

Back to Forsythia

Pi 0.00332

MarkerSeek score 40.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5616
variable_sites: 47
pi: 0.00332
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTCTTGTTCCAACTCGGTGG	CATGCCAAAGTGCTGGAAAG	50.0	50.0	58.0	57.9	752 bp	1.0	80.2
ycf1_p2	TTCTTGTTCCAACTCGGTGG	TGAACGGACCATATCGGAGA	50.0	50.0	58.0	57.9	458 bp	0.857143	74.5
ycf1_p3	CTATCCCAGGCCTCTGCTAT	TGAACGGACCATATCGGAGA	55.0	50.0	58.1	57.9	931 bp	0.857143	74.2
ycf1_p4	TATCCCAGGCCTCTGCTATC	TGAACGGACCATATCGGAGA	55.0	50.0	58.1	57.9	930 bp	0.857143	74.1
ycf1_p5	GCGCAAATGGAATGCTATCC	TGAACGGACCATATCGGAGA	50.0	50.0	57.9	57.9	945 bp	0.857143	74.1