Hotspot

rps2

LSC · 16770-17480 · 711 bp

Back to Forsythia

Pi 0.00121

MarkerSeek score 29.90

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 2
pi: 0.00121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ACGTGAGTAAAGCCAAAGCA	GGTCGGATCGATTGGTTGAA	45.0	50.0	58.0	58.0	1838-1842 bp	1.0	80.3
rps2_p2	GGAAACCCCCTATTTGCCAA	GGTCGGATCGATTGGTTGAA	50.0	50.0	58.1	58.0	1505-1509 bp	1.0	80.3
rps2_p3	ACGTGAGTAAAGCCAAAGCA	ACCCCTTGGTACCGGATTTA	45.0	50.0	58.0	58.0	1782-1786 bp	1.0	80.2
rps2_p4	ACGTGAGTAAAGCCAAAGCA	TACCCCTTGGTACCGGATTT	45.0	50.0	58.0	58.0	1783-1787 bp	1.0	80.2
rps2_p5	ACGTGAGTAAAGCCAAAGCA	TTGGATTATTGCGAGCGGAA	45.0	45.0	58.0	58.0	2002-2006 bp	1.0	80.1