Hotspot

rps15

SSC · 125514-125786 · 273 bp

Back to Forsythia

Pi 0.00209

MarkerSeek score 26.60

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 2
pi: 0.00209
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AACACCATGCATTGATGGGT	GCAACGAGAAGAATAGCAGC	45.0	50.0	58.1	57.3	1038-1047 bp	1.0	75.4
rps15_p2	GCAGCTATTCCTCCGATACG	GCAACGAGAAGAATAGCAGC	55.0	50.0	58.0	57.3	1457-1466 bp	1.0	75.3
rps15_p3	GGTCTGTGCCCCAATATCTG	GCAACGAGAAGAATAGCAGC	55.0	50.0	58.0	57.3	1350-1359 bp	1.0	75.3
rps15_p4	GATCAGCAGCTATTCCTCCG	GCAACGAGAAGAATAGCAGC	55.0	50.0	57.9	57.3	1462-1471 bp	1.0	74.8
rps15_p5	CGCCCTCAACTCGTTCTAAA	GCAACGAGAAGAATAGCAGC	50.0	50.0	57.9	57.3	1579-1588 bp	1.0	74.7