Hotspot

psaB

LSC · 39666-41870 · 2205 bp

Back to Forsythia

Pi 0.000864

MarkerSeek score 49.90

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 5
pi: 0.000864
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	GGACCCTCTCTCCATCGAAA	TACAAGGACGTGCTGTAGGA	55.0	50.0	58.5	58.1	2381 bp	1.0	75.4
psaB_p2	ATAAGAAGGCGGACCCTCTC	TACAAGGACGTGCTGTAGGA	55.0	50.0	58.6	58.1	2391 bp	1.0	74.8
psaB_p3	GGACCCTCTCTCCATCGAAA	AAGGACGTGCTGTAGGAGTA	55.0	50.0	58.5	57.8	2378 bp	1.0	74.4
psaB_p4	GGACCCTCTCTCCATCGAAA	AGGACGTGCTGTAGGAGTAA	55.0	50.0	58.5	57.8	2377 bp	1.0	74.4
psaB_p5	GGACCCTCTCTCCATCGAAA	GACGTGCTGTAGGAGTAACC	55.0	55.0	58.5	57.7	2375 bp	1.0	73.9