Hotspot

ndhA

SSC · 122041-124233 · 2193 bp

Back to Forsythia

Pi 0.00337

MarkerSeek score 54.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2193
variable_sites: 15
pi: 0.00337
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACTCAGTTACCATAGGAAACA	CCCACCGGGTTTTATCAACT	37.5	50.0	57.6	57.8	2416-2419 bp	1.0	43.5
ndhA_p2	TCATGAACTCAGTTACCATAGGA	CCCACCGGGTTTTATCAACT	39.1	50.0	56.5	57.8	2419-2422 bp	1.0	42.7
ndhA_p3	TGTTTGAGACAAGTTGTGAA	CCCACCGGGTTTTATCAACT	35.0	50.0	53.2	57.8	2348-2351 bp	1.0	40.6
ndhA_p4	TTGTTTGAGACAAGTTGTGA	CCCACCGGGTTTTATCAACT	35.0	50.0	53.2	57.8	2349-2352 bp	1.0	40.6
ndhA_p5	ACCATAGGAAACATATCGTCA	CCCACCGGGTTTTATCAACT	38.1	50.0	54.2	57.8	2405-2408 bp	1.0	40.6