Hotspot

atpF

LSC · 12812-14072 · 1261 bp

Back to Forsythia

Pi 0.00211

MarkerSeek score 49.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1261
variable_sites: 6
pi: 0.00211
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	GGCTCGAATGGTTACCATGA	AGGCAAAGTGATACAAAAAGAACT	50.0	33.3	57.7	57.3	1391-1393 bp	1.0	40.5
atpF_p2	GGCTCGAATGGTTACCATGA	ACTCTGATCGATTTTTGAGTCT	50.0	36.4	57.7	55.2	1370-1372 bp	1.0	40.5
atpF_p3	GGCTCGAATGGTTACCATGA	AGAACTCTGATCGATTTTTGAGTC	50.0	37.5	57.7	57.0	1373-1375 bp	1.0	40.5
atpF_p4	GGCTCGAATGGTTACCATGA	GAACTCTGATCGATTTTTGAGTCT	50.0	37.5	57.7	57.0	1372-1374 bp	1.0	40.5
atpF_p5	GGCTCGAATGGTTACCATGA	GGCAAAGTGATACAAAAAGAACTC	50.0	37.5	57.7	57.0	1390-1392 bp	1.0	40.5