Hotspot

accD-psaI

LSC · 61558-62258 · 701 bp

Back to Forsythia

Pi 0.0045

MarkerSeek score 41.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 8
pi: 0.0045
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAGAAGGGCTTATTCGACC	CCGGAAATACTAGGCCTACT	55.0	50.0	58.0	55.8	851-856 bp	1.0	63.4
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	CCGGAAATACTAGGCCTACT	50.0	50.0	58.8	55.8	782-787 bp	1.0	57.8
accD-psaI_p3	TCCAGAAGGGCTTATTCGAC	CCGGAAATACTAGGCCTACT	50.0	50.0	57.0	55.8	852-857 bp	1.0	55.9
accD-psaI_p4	GCTCCACGCTTTCTTTCCT	CCGGAAATACTAGGCCTACT	52.6	50.0	58.1	55.8	782-787 bp	1.0	55.3
accD-psaI_p5	TCCACGCTTTCTTTCCTTTGA	CCGGAAATACTAGGCCTACT	42.9	50.0	58.1	55.8	780-785 bp	1.0	55.3