Hotspot

trnG(GCC)-trnfM(CAU)

LSC · 38849-39017 · 169 bp

Back to Forsythia

Pi 0.0189

MarkerSeek score 56.70

Primer available yes

Label trnG(GCC)-trnfM(CAU)

Feature Metadata

label_name: trnG(GCC)-trnfM(CAU)
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 8
pi: 0.0189
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG(GCC)-trnfM(CAU)_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	234-237 bp	1.0	82.3
trnG(GCC)-trnfM(CAU)_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	235-238 bp	1.0	82.3
trnG(GCC)-trnfM(CAU)_p3	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	270-273 bp	1.0	82.0
trnG(GCC)-trnfM(CAU)_p4	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	271-274 bp	1.0	82.0
trnG(GCC)-trnfM(CAU)_p5	ATGCGGATATGGTCGAATGG	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	57.9	58.0	311-314 bp	1.0	81.3