Hotspot

psbZ-trnG(GCC)

LSC · 38481-38778 · 298 bp

Back to Forsythia

Pi 0
MarkerSeek score 34.90
Primer available yes
Label psbZ-trnG(GCC)

Feature Metadata

label_name
psbZ-trnG(GCC)
feature_type
igs
region
LSC
length_bp
298
variable_sites
0
pi
0
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG(GCC)_p1 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 464-470 bp 1.0 78.2
psbZ-trnG(GCC)_p2 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 465-471 bp 1.0 78.2
psbZ-trnG(GCC)_p3 GCTTCTCCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.3 58.1 462-468 bp 1.0 77.8
psbZ-trnG(GCC)_p4 GCTTCTCCTGATGGTTGGTC GAATCGAACCCGCATCTTCT 55.0 50.0 58.3 58.1 463-469 bp 1.0 77.8
psbZ-trnG(GCC)_p5 GGGTTAGTCTTTCTGGTGGG AATCGAACCCGCATCTTCTC 55.0 50.0 57.5 58.1 399-405 bp 1.0 76.0